Serum Free Cryoprotective Media With DMSO

Description

Background

During cryopreservation cooling aqueous solutions below the equilibrium freezing point will inevitably result in the formation of ice crystals in the extracellular media. PurMa™ Serum Free Cryoprotective Media With DMSO allows the cryoprotective agent to get inside the cells instantly and lower cell death because of penetration / freezing in the absence of any serum or animal product. That will inevitably cause a decrease in the concentration of water, which has two key consequences:

1. Osmotic gradient forms across the cell membrane, which causes “cell dehydration”, and
2. Ice formation and abnormal concentration of solutes in intracellular matrix, osmotic shock, and toxicity.

More Importantly, we always manufacture  our cryoprotective media using our most sophisticated water , for more details, Click here:

PurMa™ Ultra-Pure Cell Culture Suited Water

Serum in Cryoprotective Media

Serum and its components perform a crucial role in cells viability as well as recovery during cryopreservation. Nevertheless, there are known disadvantages of serum in cryoprotective media:

1. The inability to fully sterilize them.
2. Moreover, unknown composition.
3. And finally, the batch-to-batch variation which results in inconsistency in the rate of survival, recovery, and viability in cryopreservation. Additionally, this is more crucial for cells sensitive to cryopreservation.
4. Therefore, a chemically defined cryoprotective media resolves several issues, including the above-mentioned restrictions.

Two Major Benefit

1. PurMa^TM Serum Free Cryoprotective Medium (SFCM) prevents cell hydration and formation of ice crystals, therefore is an excellent tool for short- and long-term cryopreservation.
2. PurMa^TM Serum Free Cryoprotective Medium (SFCM) contains DMSO.

At PurMa Biologics, with three decades of experience, we have evaluated different combinations of synthetic polymers that can replace serum. We have successfully developed a fully defined cryoprotective media which is free of animal products .

DMSO benefits cryoprotection and cryoprotective agent in several ways

1. Provides strong molecular stability.
2. Besides, facilitates a high non-conductor constant.
3. It has the basic property of salt solubility, particularly for anions. Additionally, it has a propensity to act as the acceptor atom in hydrogen bonding.
4. Especially, DMSO makes a  hydrogen bond with water which is 30% stronger than between two water molecules.

Special Features

• Ready-to-Use medium.
• Each Lot of PurMa^TM Serum Free Cryoprotective Medium (SFCM) goes through pathogens and sterility evaluations.
• We evaluate each Lot for survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), as well as for the rate of recovery by Alamar Blue test.
• PurMa^TM SFCM results in the lowest cell loss during freezing and thawing as well as the highest cell recovery. Moreover, it provides over 66% survival and recovery rate after long term cryopreservation.
• Sterile (Passed through 0.2µm filters).
• Moreover, PurMa^TM SFCM provides an exceptional  cryopreservation for primary and commercial cell lines
• Suitable for clinical applications.

Guideline

for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines

Reference

1. Serum-free cryopreservation of porcine hepatocytes. Müller et al. Cell Tissue Res. 2004 Jul;317(1):45-56. doi: 10.1007/s00441-004-0894-6. Epub 2004 Jun 5. PMID: 15185140.
2. Serum- and xeno-free cryopreservation of human umbilical cord tissue as mesenchymal stromal cell source. Shimazu et al. Cytotherapy. 2015 May;17(5):593-600. doi: 10.1016 /j.jcyt.2015.03.604. PMID: 25881518.

Parameter	Specification
Appearance	Red liquid
pH	7.4 ± 0.1
Osmolality	275-360 mOsm/L
Endotoxin	NMT< 2EU/mL
Mycoplasma	Negative
Suitability	Suitable for mammalian cell culture
Sodium pyruvate	NA
Indicator	NA
Sterility Tested	Sterile filtered using 0.22 µm filter
Form	Liquid
Shipping Condition	Dry Ice

 

Learn more: What Is Cryoprotective Media?