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Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer

Advanced Minimum Essential Media (A-MEM) in HBSS

(36 customer reviews)

Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction.  Additionally, Advanced MEM needs 2-5% FBS and provides more consistency.

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SKU: N/A Categories: Cell Culture Media, Minimum Essential Media (MEM) In Hanks’ Buffer Tags: Advanced, Advanced MEM-α, Cell Culture, Hanks' Balanced Salt Solution, Hanks’ Buffer, HBSS, Human Transferrin, L-Alanyl-L-Glutamine, MEM-α, Recombinant Insulin, Serum Reduction

Description

Background

PurMaTM Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. Consequently, this results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in A-MEM. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.

Contents

Advanced MEM in HBSS Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-MEM incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM (CD-A-MEM) is the outcome of decades of innovative cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced MEM (HBSS) Formulation in Hanks’ Buffer 

References

  1. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya T, Takahashi K. J Anat. 1987 Jun;152:209-13. PMID: 3654371
  2. Long-term organ culture of hamster cheek pouch mucosa. Mock D, Main JH. J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x. PMID: 820846
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

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