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Prokaryotic Cell Culture Suited Antibiotics
PurMa Biologics offers Prokaryotic Cell Culture Suited Antibiotics. Altogether, this collection of antibiotics prevent contamination in the most common Prokaryote cells. To gain a better understanding of the main function of each antibiotic, as well as the target cells, mechanism of function and the corresponding literature, please visit the booklet below.
Description
Background
PurMa Biologics offers Prokaryotic Cell Culture Suited Antibiotics. Altogether, this collection of antibiotics prevents contamination in the most common Prokaryote cells. To keep these cells free of contamination, antibiotics should mostly function as aminoglycosides and target the cell wall. The main function of each antibiotic as well as the target cells, mechanism of function and the corresponding literature has been documented in below booklet:PurMa™ Prokaryotes Antibiotics booklet
Special Feature
- This collection of antibiotics does not contain any PH manipulating ingredients.
- Besides, after preparation, we sterilize the antibiotic mix by passing through 0.1 µm filters.
- Furthermore, we manufacture these antibiotics in PurMa™ Ultra-Pure Cell Culture Suited Water. Click here for more details: PurMa™ Ultra-Pure Cell Culture Suited water
- We pack our antibiotic mix under N2.
Formulations and Methods of Preparation
For complete formulation and method of preparation, Click here: Formulations and Methods of preparation
References
- Mechanism of action of aminoglycoside antibiotics. Binding studies of tobramycin and its 6′-N-acetyl derivative to the bacterial ribosome and its subunits. Le Goffic F, Capmau ML, Tangy F, Baillarge M. Eur J Biochem. 1979 Dec;102(1):73-81. doi: 10.1111/j.1432-1033.1979.tb06264.x.
- Review for Analytical Methods for the Determination of Sodium Cephalothin. Rugani KS, Kogawa AC, Salgado HRN. Crit Rev Anal Chem. 2019;49(2):187-194. doi: 10.1080/ 10408347 .2018. 1506697. Epub 2018 Dec 5.
Additional information
| Antibiotic | Ampicillin (1000X), 50 mg/ml, Cefotaxime (100X), 10 mg/ml, Cephalothin sodium salt (100X), 10 g/ml, Chloramphenicol (1000X), 50 mg/ml, Erythromycin (100X), 10 g/ml, G 418, 10 mg/ml, Gentamicin Sulfate (100X), 500 mg/ml, Kanamycin Sulfate (1000X), 50 mg/ml, Neomycin Sulfate (1000X), 50 mg/ml, Polymyxin B Sulfate (100X), 0.5 mg/ml, Spectinomycin dihydrochloride pentahydrate (100X), 2 mg/ml |
|---|---|
| Size | 20 ml, 50 ml, 100 ml |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
Frequently asked questions
What working concentration should I use for Prokaryotic Cell Culture Suited Antibiotics?
Most antibiotic mixes are supplied as a 100X stock, giving a 1 in 100 dilution into complete medium. Check the Certificate of Analysis for the specific lot, since concentration varies between formulations.
Should antibiotics be used routinely in cell culture?
Most quality guidance advises against it. Continuous use masks low level contamination, allows poor aseptic technique to go unnoticed, and can select for resistant organisms. They are appropriate for high risk work such as primary tissue rather than as a standing habit.
Will this prevent yeast or fungal contamination?
Not unless it contains an antimycotic. Penicillin and streptomycin act on bacteria only. Preventing fungi or yeast requires amphotericin B or a similar antimycotic, and even then technique matters more than chemistry.
How should Prokaryotic Cell Culture Suited Antibiotics be stored?
Frozen at minus 20 degrees C and aliquoted on first thaw. Activity declines with repeated freeze and thaw cycles, which shows up as contamination creeping back into cultures that were previously clean.
Is it sterility and endotoxin tested?
Yes. Every lot is filtered and tested, and a Certificate of Analysis is issued per lot and available on request.
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