- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Dialyzed Fetal Horse Serum (DFHS)
Fetal horse serum dialyzed to remove small molecules, for experiments that need a more defined environment.
- Reduces low-molecular-weight components for defined-media studies
- Improves consistency across cell culture experiments
Description
Background
Animal serum contains many undefined components. PurMa™ Dialyzed Fetal Horse Serum (DFHS) provides more consistency among the cell culture experiments. This is especially true in studies requiring a more defined environment of small molecules. The dialysis process eliminates/ reduces the concentration of low molecular weight components. That includes antibiotics, nucleotides, amino acids, hormones, salts, and various small proteins in serum.
PurMaTM Dialyzed FHS is Free of Tetracycline and other Antibiotics.
PurMa™ Dialyzed FHS (DFHS)
- DFHS has been dialyzed using the 3500 Dalton (3.5 kDa) dialysis tube. The dialysis is carried out against 10X 10-liter PBS (pH 7.4). At the end, the dialyzed FHS is filtered three times (0.1 µM filters).
- PurMa™ Dialyzed Fetal Horse Serum (DFHS) is of USA origin.
- The parent DFHS has been gamma irradiated.
- Following the dialysis, the FHS goes through heat inactivation process.
- Following the dialysis, the FHS goes through vigorous analysis and will get a certificate of analysis.
- Moreover, PurMa™ Dialyzed Fetal Horse Serum (DFHS) is free of Mycoplasma, steroids and hormones and other pathogens.
- PurMa™ DFHS is free of endotoxin and also contains an acceptable level of hemoglobin (20 mg/Dl).
- Due to the loss of small growth factors and other essential elements below the size of 3.5 kDa we recommend using 15-20% DFHS in your media to ensure cultured cell wellbeing.
Further Instructions
On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.
Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums
References
- Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
- Higher percentage of horse serum in culture media blocks attachment of PC12 cells. Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Learn more: What Is Horse Serum?
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
What our customers say about PurMa Biologics
Feedback from labs we supply, across our full catalogue.
PurMa products are of excellent quality and highly reliable.
I've ordered cell culture media multiple times. Great prices and excellent customer service. The ordering process was smooth, and everything arrived as expected. I'll definitely be purchasing from them again.
We buy all of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
We have been working with PurMa biologics for the past several years, we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum. We appreciate the high quality and reasonable price, and great customer service.
We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and... Always great quality, fast processing and shipping. We are totally satisfied.
Very fast, efficient, and economic service. Great for new PIs like me.
Frequently asked questions
How should I store and thaw Dialyzed Fetal Horse Serum (DFHS)?
Store at minus 20 degrees C for long term use. Thaw overnight at 2 to 8 degrees C and then bring to room temperature with occasional gentle swirling, rather than plunging straight into a 37 degree bath. Aliquot after the first thaw, because repeated freeze and thaw cycles damage growth factors and cause cryoprecipitate.
Do I need to heat inactivate it?
Only for applications sensitive to complement, such as some immunological assays. Heat inactivation at 56 degrees C for 30 minutes also reduces some growth factors, so for routine culture most labs use serum without it. Test both if your cells are sensitive.
What testing is performed on each lot?
Each lot is tested for sterility, mycoplasma and endotoxin, along with a biochemical profile and haemoglobin. Results are reported on the Certificate of Analysis for that specific lot.
What are the flakes or particles in my thawed serum?
Almost always cryoprecipitate, which is fibrin and lipoprotein coming out of solution during freezing and thawing. It is normal, does not indicate contamination, and does not affect performance. It can be removed by filtration if it interferes with your assay.
Can I reserve a specific lot for repeat orders?
Yes. Labs that validate a lot for a sensitive application can reserve volume from it. Request lot reservation through the bulk pricing form on this page and state the volume and timescale you need.
Related products
-
- Sale!
- Fetal Bovine Serum, Bovine Serums
Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)
- Price range: $111.23 through $545.69
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Fetal Bovine Serum, Horse Serums
Lipid-Depleted Fetal Horse Serum (LD-FHS)
- Price range: $652.65 through $1989.69
- Select options This product has multiple variants. The options may be chosen on the product page
-
- Fetal Bovine Serum, Horse Serums
PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS)
- Price range: $495.36 through $1089.69
- Select options This product has multiple variants. The options may be chosen on the product page
Reviews