Advanced Eagle’s Minimal Essential Medium (A-EMEM)
Advanced Eagle’s Minimal Essential Medium (A-EMEM) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-EMEM needs 2-5% FBS and provides more consistency.
Description
Background
PurMaTM Advanced Eagle’s Minimal Essential Medium (A-EMEM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-EMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced EMEM Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-EMEM incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Eagle’s Minimal Essential Medium (A-EMEM) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced EMEM (CD-A-EMEM) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced EMEM Formultaion
References
- Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, Dixon OW, Lizzio EF. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
- Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, Sedwick WD, Brown OE, Laszlo J. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-EMEM Standard Formulation, A-EMEM w/o L-Alanyl-L-Glutamine, Advanced EMEM w/o Phenol Red, A-EMEM w/o Sodium Bicarbonate, A-EMEM w/o Sodium Pyruvate, A-EMEM w/o Glucose, A-EMEM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-EMEM w/o Phenol Red, w/o Sodium Bicarbonate, A-EMEM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-EMEM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-EMEM High Glucose (4.5 g/L), A-EMEM High Sodium Bicarbonate (2.438 g/L), A-EMEM High Glucose (4.5 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM 15 mM HEPES (3.6 g/L), Advanced EMEM 25 mM HEPES (5.9 g/L), Advanced EMEM w/o Sodium Pyruvate, w/o Phenol Red, A-EMEM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), A-EMEM w/o Phenol Red, with 25 mM HEPES (5.9 g/L), A-EMEM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
18 reviews for Advanced Eagle’s Minimal Essential Medium (A-EMEM)
Only logged in customers who have purchased this product may leave a review.
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azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 21-Feb-2025 15:30:00
Ease of placing the order: ‘not given’
Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 24-Feb-2025 13:50:14
Ease of placing the order: ‘not given’
lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 27-Feb-2025 17:42:44
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 16:31:44
Ease of placing the order: ‘not given’
michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 17:29:13
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 10-Mar-2025 14:32:38
Ease of placing the order: ‘not given’
jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5
hyeungk1968@gmail.comthe –
Review: I used Lipid Free horse serum of PurMa Biologics.
The quality is great!
Have you purchased: Yes
Time of submission: 17-Mar-2025 12:09:58
Ease of placing the order: 5/5
jaya-krishnan@omrf.org –
Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
Have you purchased: Yes
Time of submission: 17-Mar-2025 14:22:58
Ease of placing the order: 4/5
tayyab-afzal@omrf.org –
Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
Have you purchased: Yes
Time of submission: 18-Mar-2025 10:01:50
Ease of placing the order: 5/5
Fitzkr@gmail.com –
Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
Always great quality, fast processing and shipping.
We are totally satisfied.
Have you purchased: Yes
Time of submission: 22-Mar-2025 10:47:00
Ease of placing the order: 5/5
vmadka@ouhsc.edu –
Review:
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:02:40
Ease of placing the order: 4/5
Anupam-Mandal@ouhsc.edu –
Review: Excellent
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:05:43
Ease of placing the order: 5/5
rohan-varshney@ouhsc.edu –
Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:06:38
Ease of placing the order: 5/5