Advanced Leibovitz’s L-15 Medium

Advanced Leibovitz’s L-15 Medium contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Furthermore, Advanced Leibovitz’s L-15 needs 2-5% FBS and additionally provides more consistency.

Description

Advanced Leibovitz’s L-15 Medium

Background

PurMaTM Advanced Leibovitz’s L-15 Medium contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. In A-Leibovitz’s L-15, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Contents

Advanced Leibovitz’s L-15 Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced Leibovitz’s L-15 Medium is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced Leibovitz’s L-15 (CD-A-Leibovitz’s L-15) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced Leibovitz’s L-15 Formulation

References

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A-Leibovitz's L-15 Standard Formulation, A-Leibovitz's L-15 w/o L-Alanyl-L-Glutamine, A-Leibovitz's L-15 w/o Phenol Red, A-Leibovitz's L-15 w/o Sodium Pyruvate, A-Leibovitz's L-15 w/o Galactose, A-Leibovitz's L-15 w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-Leibovitz's L-15 w/o L-Alanyl-L-Glutamine, w/o Sodium Pyruvate, A-Leibovitz's L-15 with High Galactose (4.5 g/L), A-Leibovitz's L-15 with 15 mM HEPES (3.6 g/L), A-Leibovitz's L-15 with 25mM HEPES (5.9 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Advanced Leibovitz’s L-15

 

SDS

SDS

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