Description

Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer

Background

PurMa^TM Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. Consequently, this results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in A-MEM. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.

Contents

Advanced MEM in HBSS Contains:

• includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
• A-MEM incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

• PurMa^TM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
• PurMa^TM Insulin Recombinant
• Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM (CD-A-MEM) is the outcome of decades of innovative cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced MEM (HBSS) Formulation in Hanks’ Buffer 

References

1. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya T, Takahashi K. J Anat. 1987 Jun;152:209-13. PMID: 3654371
2. Long-term organ culture of hamster cheek pouch mucosa. Mock D, Main JH. J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x. PMID: 820846