Advanced Roswell-Park Memorial Institute 1640 (A-RPMI-1640)
Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-RPMI-1640 needs 2-5% FBS and provides more consistency.
Description
Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640)
Background
PurMaTM Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-RPMI-1640, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-RPMI-1640 incorporates the elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Additionally, Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) is a significant step toward a chemically defined form of this media. Chemically Defined Advanced RPMI-1640 (CD-A-RPMI-1640) is the outcome of decades of cutting-edge research. It will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced RPMI-1640 Formulation
References
- Culture of normal human leukocytes. Moore GE, et al. 1967 Feb 20;199(8):519-24. PMID: 4960081
- Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. Dunne HW, et al. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.
- Long-term culture of human leukaemic leukocytes. Pössnerová V, et al. 1970;17(5):513-23. PMID: 5274436
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-RPMI-1640 Standard Formulation, A-RPMI-1640, Standard Formulation (ATCC formulation), A-RPMI-1640 w/o L-Alanyl-L-Glutamine, A-RPMI-1640 w/o Phenol Red, A-RPMI-1640 w/o Sodium Bicarbonate, A-RPMI-1640 w/o Glucose, A-RPMI-1640 w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-RPMI-1640 w/o Phenol Red, w/o Sodium Bicarbonate, A-RPMI-1640 w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-RPMI-1640 With Low Glucose (1 g/L), A-RPMI-1640 With High Sodium Bicarbonate (3.7 g/L), A-RPMI-1640 With 15 mM HEPES (3.6 g/L), A-RPMI-1640 With 25 mM HEPES (5.9 g/L), A-RPMI-1640 With 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (3.7 g/L), A-RPMI-1640 With 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (3.7 g/L, A-RPMI-1640 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), A-RPMI-1640 w/o Phenol Red, with 25 mM HEPES (5.9 g/L), A-RPMI-1640 w/o Phenol Red, High Sodium Bicarbonate (3.7 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]Formulation
SDS
SDS
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