Description

Basal Medium Eagle (BME) in Hanks’ Buffer

Background

Basal Medium Eagle (BME) in Hanks’ Buffer originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Markedly, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). PurMa Biologics presently manufactures BME with Earle’s for use in a CO₂ incubator, or with Hanks’ (HBSS) salts for use without CO₂.

Ingredients

As the composition, this media contains HBSS buffer

HBSS buffer contains:

1. Potassium Chloride
2. Potassium Phosphate, monobasic
3. Sodium Bicarbonate
4. Sodium Chloride
5. And lastly, Sodium Phosphate, dibasic

Application

PurMa Biologics manufactures 117 types BME in HBSS buffer. This media has been successfully used for variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as  primary rat astrocytes.

Formulation

For complete formulation click, or alternatively  look at the “ Formulation Tab”:  BME with Hanks’ Buffer (HBSS) Formulation

References

1. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS et al. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
2. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971.
3. Differential cytopathogenicity accompanying Mycoplasma pneumoniae infection of human lung fibroblasts maintained in newborn bovine serum or fetal bovine serum. Upchurch et al. In Vitro. 1983 Mar;19(3 Pt 1):203-9. doi: 10.1007/BF0 2618060. PMID: 6187665.