Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)
Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is commonly used worldwide. Moreover, DMEM F-12 contains more nutrition and offers more buffering capacity. Therefore, this medium provides more growth and maintenance ability.
Description
DMEM F-12
Background
Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) provides superior quality, consistency, and great control in mammalian cell culture. DMEM F-12 is preferred for MDCK, glial cells, fibroblasts, human endothelial cells, as well as rat fibroblasts. In general, DMEM: F-12 is more enriched but contains the following major changes compared to DMEM:
Ingredients
- It contains 2.5 mM L-glutamine
- 0.5 mM sodium pyruvate
- Moreover, contains 30mM NaHCO3 (2.438g/L)
- High glucose
- Non-Essential Amino Acids
- also, contains Sodium pyruvate
- Phenol Red
- L-glutamine
Formulation
For complete formulation click here: DMEM F-12 Formultaion
References:
- Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016 /0042-68 22(59)90043-1. PMID: 13669362.
- Plaque production by the polyoma virus. DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 1366936.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | DMEM F-12 Standard Formulation, DMEM F-12 Standard Formulation, ATCC Formulation, DMEM F-12 w/o Glutamine, DMEM F-12 w/o Phenol Red, DMEM F-12 w/o Sodium Bicarbonate, DMEM F-12 w/o Sodium Pyruvate, DMEM F-12 w/o Glucose, DMEM F-12 w/o Lipoic Acid, DMEM F-12 w/o Glutamine, w/o Phenol Red, DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, DMEM F-12 w/o Sodium Bicarbonate, w/o Sodium Pyruvate, DMEM F-12 w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, DMEM F-12 Low Glucose (1.5 g/L), DMEM F-12 Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 15 mM HEPES (3.6 g/L), DMEM F-12 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 w/o Sodium Pyruvate, w/o phenol Red, DMEM F-12 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), DMEM F-12 w/o Phenol Red, Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 High Glucose (4.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]Formulation
SDS
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