Description

Dulbecco’s Phosphate Buffered Saline (DPBS)

Background

Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution. It is particularly used to maintain viability of cells for a short time instead of promoting their growth. During interval period we maintain osmotic balance and physiological pH to protect cellular integrity. So, DPBS serves as an isotonic saline solution or buffer for washing cells and tissues like in chromatographic procedures where incubation is not necessary.

Grades of Phosphate Buffered Saline (PBS)

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

we finally sterilize the buffers by autoclaving the solution and and packding  in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2

The Endotoxin Removing Procedure from Buffer

PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer  through the PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B   to an endotoxin-specific absorbent. we finally  eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

General Feature

• PurMa Biologics manufactures this buffer in two pH: 7.4 ± 0.01 and 7.2 ± 0.01 at 25°C.
• We rigorously sterilize it by 0.22µm membrane.
• Additionally, it is a cost-effective balanced salt solution used for a variety of biochemical applications.
• PurMa Biologics packs all our buffer under N_2, as a result, the pH wont change over the time before opening the bottle.

Specific Feature

• Cell Culture Suited
• We deplete  Mg²⁺ and Ca²⁺ ions
• Our manufacturing process removes  RNAse /DNase
• Free of Proteases.
• PurMa Biologics  rigorously test for contaminating,  nonspecific endonuclease, exonuclease, as well as  RNase activity.

For complete formulation, Click here:DPBS Formulation

 References:

1.  Plaque formation and isolation of pure lines with poliomyelitis viruses. Dulbecco et al.J Exp Med.; 1954 Jan 31 99(2): 167–182. doi: 10.1084/jem.99.2.167. PMID: 13130792.
2. Deep freezing of sheep embryos. Willadsen Set al. J Reprod Fertil. 1976 Jan;46(1):151-4. doi: 10.1530/jrf.0.0460151. PMID: 1271336.