Kaighn's Modification of Ham's F-12-K Medium

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Kaighn's Modification of Ham's F-12 Medium (Ham's F-12K)

Kaighn’s Modification of Ham’s F-12-K Medium

$28.31 – $547.80

Kaighn’s Modification of Ham’s F-12-K Medium is one of the widely used media. PurMa Biologics manufactures 96 types of this media. Moreover, (Ham’s F-12K) contains  components not found in older media, such asputrescine thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate

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Description

Kaighn’s Modification of Ham’s F-12-K Medium

Background

Kaighn’s Modification of Ham’s F-12-K Medium is a modified version of Ham’s F-12 medium. originally,  Ham’s F-12K (Kaighn’s) was used for primary cells such as human oocytes and hepatocytes, as well murine and chicken liver cells in a lower serum environment. but, however, recently this media is used for a much larger variety of cells.

Moreover, Ham’s F-12K (Kaighn’s) Medium contains a variety of components not found in older media, such as putrescine thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate.

Ingredients:

F-12K Medium also contains:

–  Low level of glucose (1.26 g/ liter)

–  Sodium bicarbonate (2.5 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed).

– Additionally, PurMa Biologics also offers F-12K Medium with different level of  sodium bicarbonate : 1.5 g/L (Cat # P3S057108) as well as, 3.7g/L (Cat # P3S032108)

–  2 mM L-glutamine (292 mg/L)’

– A higher concentration of amino acids, vitamins, inositol and choline are present in this medium.

Why PurMa Biologics? Why we stand behind our Cell Culture Media:

Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.  Also, we utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.

We routinely manufacture over 1500 media, which is the most comprehensive collection of cell and tissue culture media. Considering the highest quality, our prices are extremely reasonable.

Application of Ham’s F-12K:

This media has elements that prevent clumping, therefore, it is ideal for culturing primary as well as commercial cell lines, for instance normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells.

Formulation:

For complete formulation click here: Ham’s F-12K Formulation

References:

  1. Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss et al. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
  2. Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using BALB/c 3T3 mouse embryo fibroblasts. Riss et al. In Vitro Cell Dev Biol. 1988 Nov;24(11):1099-106. doi: 10.1007/BF02620811.
  3. Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium. Fong et al. Diabetes. 1981 Dec;30(12):1022-8. doi: 10.2337/diab.30.12.1022. PMID: 6273246
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Formulation

Ham’s F-12K Formulation

Quality Control

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    Background

    Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific  requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer for use in a CO2 incubator, or with Hanks' salts for use without CO2.

    PurMa™ MEM media in Hank’s buffer includes Hank's buffer

    Hanks buffer, it contains:
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    2. Potassium Phosphate, monobasic
    3. Sodium Bicarbonate
    4. Sodium Chloride
    5. Sodium Phosphate, dibasic

    Additionally, MEM in Hanks’ Buffer contains:

    • Low level of glucose (1.00 g/ liter)
    • Sodium bicarbonate (0.35 g/L). If , however, higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
    • 2 mM L-glutamine (292 mg/L)
    Moreover, PurMa Biologics manufactures 122 types of MEM.

    Why PurMa Biologics? Why we stand behind our cell culture media

    Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

    Applications

    MEM has been used for the cultivation of a wide variety of cells grown in monolayers attached or in suspension cell lines such as HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts as well as primary rat astrocytes. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% fetal bovine serum (FBS).

    Formulation

    Complete formulation click here as well as in Formulation Tab: MEM (HBSS) Formulation in Hanks' Buffer

    References

    1. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
    2. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya et al. J Anat. 1987 Jun;152:209-13. PMID: 3654371
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filter_opacity_hover="100" filter_blur_hover="0" transform_type="regular" transform_hover_element="self" transform_scale_x="1" transform_scale_y="1" transform_translate_x="0" transform_translate_y="0" transform_rotate="0" transform_skew_x="0" transform_skew_y="0" transform_scale_x_hover="1" transform_scale_y_hover="1" transform_translate_x_hover="0" transform_translate_y_hover="0" transform_rotate_hover="0" transform_skew_x_hover="0" transform_skew_y_hover="0" transform_origin="" transition_duration="300" transition_easing="ease" transition_custom_easing="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset="" last="true" border_position="all" first="true"][fusion_text columns="" column_min_width="" column_spacing="" rule_style="" rule_size="" rule_color="" hue="" saturation="" lightness="" alpha="" content_alignment_medium="" content_alignment_small="" content_alignment="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" sticky_display="normal,sticky" class="" id="" margin_top="" margin_right="" margin_bottom="" margin_left="" fusion_font_family_text_font="" fusion_font_variant_text_font="" font_size="" line_height="" letter_spacing="" text_transform="" text_color="" animation_type="" animation_direction="left" animation_color="" animation_speed="0.3" animation_delay="0" animation_offset=""]

    Advanced Eagle’s Minimal Essential Medium (A-EMEM)

    Background

    PurMaTM Advanced Eagle’s Minimal Essential Medium (A-EMEM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-EMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

    Contents

    Advanced EMEM Contains:

    • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
    • A-EMEM incorporates elements that significantly reduce the need for a serum for cell culture.

    Crucial Ingredients:

    • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
    • PurMaTM Insulin Recombinant
    • Trace of other elements which mimic the elements in fetal bovine serum.
    Consequently, Advanced Eagle’s Minimal Essential Medium (A-EMEM) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced EMEM (CD-A-EMEM) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

    Formulation

    Complete formulation is available here: Advanced EMEM Formultaion

    References

    1. Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, Dixon OW, Lizzio EF. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
    2. Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, Sedwick WD, Brown OE, Laszlo J. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH  7.2 ± 0.1
    Osmolality  275-360 mOsm/L
    Endotoxin  NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive  Sodium pyruvate
    Indicator  Phenol red
    Mycoplasma Detection Negative
    Sterility Tested  Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

  • Basal Medium Eagle (BME) in Hanks’ Buffer

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    Basal Medium Eagle (BME) in Hanks’ Buffer

    Background

    Basal Medium Eagle (BME) in Hanks’ Buffer originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Markedly, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). PurMa Biologics presently manufactures BME with Earle’s for use in a CO2 incubator, or with Hanks' (HBSS) salts for use without CO2.

    Ingredients

    As the composition, this media contains HBSS buffer HBSS buffer contains:
    1. Potassium Chloride
    2. Potassium Phosphate, monobasic
    3. Sodium Bicarbonate
    4. Sodium Chloride
    5. And lastly, Sodium Phosphate, dibasic

    Application

    PurMa Biologics manufactures 117 types BME in HBSS buffer. This media has been successfully used for variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as  primary rat astrocytes.

    Formulation

    For complete formulation click, or alternatively  look at the “ Formulation Tab”:  BME with Hanks' Buffer (HBSS) Formulation

    References

    1. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS et al. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
    2. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971.
    3. Differential cytopathogenicity accompanying Mycoplasma pneumoniae infection of human lung fibroblasts maintained in newborn bovine serum or fetal bovine serum. Upchurch et al. In Vitro. 1983 Mar;19(3 Pt 1):203-9. doi: 10.1007/BF0 2618060. PMID: 6187665.
    [/fusion_text][fusion_table fusion_table_type="2" fusion_table_rows="13" fusion_table_columns="2" margin_top="30px" margin_right="" margin_bottom="" margin_left="" hide_on_mobile="small-visibility,medium-visibility,large-visibility" class="" id="" animation_type="" animation_direction="left" animation_color="" hue="" saturation="" lightness="" alpha="" animation_speed="0.3" animation_delay="0" animation_offset=""]
    Parameter Specification
    Appearance Red, clear liquid
    pH 7.2 ± 0.1
    Osmolality 275-360 mOsm/L
    Endotoxin NMT< 2EU/mL
    Mycoplasma Negative
    Suitability  Suitable for mammalian cell culture
    Additive N/A
    Indicator Phenol red
    Mycoplasma Detection Negative
    Sterility Tested Sterile filtered using 0.22 µm filter
    Form Liquid
    Shipping Condition  Room temperature
    [/fusion_table][/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed Read more

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