Kaighn’s Modification of Ham’s F-12 Medium is a modified version of Ham’s F-12 medium. Ham’s F-12K (Kaighn’s) was originally used for primary cells such as human oocytes and hepatocytes, as well murine and chicken liver cells in a lower serum environment.

Ham’s F-12K (Kaighn’s) Medium contains a variety of components not found in older media, such as putrescine (therefore the media smells after incubated with cells after 48 hours), thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These compensated for supplemental serum. But the addition of 10 % FBS is necessary. PurMaTM F-12K Medium contains no proteins or growth factors and therefore should be supplemented with FBS.  PurMa TM F-12K Medium also contains:

–  Low level of glucose (1.26 g/ liter)

–  Sodium bicarbonate (2.5 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed).

– PurMa Biologics also offers F-12K Medium with low sodium bicarbonate (1.5 g/L) (Cat # P3S057108) as well as high sodium bicarbonate (3.7g/L) (Cat # P3S032108)

–  2 mM L-glutamine (292 mg/L)’

– A higher concentration of amino acids, vitamins, inositol and choline are present in this medium.

PurMa Biologics manufactures 96 types of Ham’s F-12K which accommodate any form of this medium. We recommend contacting our friendly customer service & technical support about your exact need for Ham’s F-12K   media.

Why PurMa Biologics? Why we stand behind our cell culture media:

  1. Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.
  2. We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media.
  3. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.
  4. Considering the highest quality, our prices are extremely reasonable.

Application of Ham’s F-12K  

This media has elements that prevent clumping the cells which is a necessity for performing analysis on these cells. Ham’s F-12K has been formulated for use in a 5% carbon dioxide atmosphere and is not recommended for higher and lower percentages of CO2. It utilizes a bicarbonate buffering system. Scientists in PurMa Biologics have successfully evaluated Ham’s F-12K  medium for the culture of vast varieties of primary as well as commercial cell lines including: normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells. Ham’s F-12K   Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Formulation: for complete formulation click here: Ham’s F-12K Formulation

Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature