Kaighn’s Modification of Ham’s F-12-K Medium

Kaighn’s Modification of Ham’s F-12-K Medium is one of the widely used media. PurMa Biologics manufactures 96 types of this media. Moreover, (Ham’s F-12K) contains  components not found in older media, such asputrescine thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate

Description

Kaighn’s Modification of Ham’s F-12-K Medium

Background

Kaighn’s Modification of Ham’s F-12-K Medium is a modified version of Ham’s F-12 medium. originally,  Ham’s F-12K (Kaighn’s) was used for primary cells such as human oocytes and hepatocytes, as well murine and chicken liver cells in a lower serum environment. but, however, recently this media is used for a much larger variety of cells.

Moreover, Ham’s F-12K (Kaighn’s) Medium contains a variety of components not found in older media, such as putrescine thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate.

Ingredients:

F-12K Medium also contains:

–  Low level of glucose (1.26 g/ liter)

–  Sodium bicarbonate (2.5 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed).

– Additionally, PurMa Biologics also offers F-12K Medium with different level of  sodium bicarbonate : 1.5 g/L (Cat # P3S057108) as well as, 3.7g/L (Cat # P3S032108)

–  2 mM L-glutamine (292 mg/L)’

– A higher concentration of amino acids, vitamins, inositol and choline are present in this medium.

Why PurMa Biologics? Why we stand behind our Cell Culture Media:

Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.  Also, we utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.

We routinely manufacture over 1500 media, which is the most comprehensive collection of cell and tissue culture media. Considering the highest quality, our prices are extremely reasonable.

Application of Ham’s F-12K:

This media has elements that prevent clumping, therefore, it is ideal for culturing primary as well as commercial cell lines, for instance normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells.

Formulation:

For complete formulation click here: Ham’s F-12K Formulation

References:

  1. Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss et al. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
  2. Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using BALB/c 3T3 mouse embryo fibroblasts. Riss et al. In Vitro Cell Dev Biol. 1988 Nov;24(11):1099-106. doi: 10.1007/BF02620811.
  3. Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium. Fong et al. Diabetes. 1981 Dec;30(12):1022-8. doi: 10.2337/diab.30.12.1022. PMID: 6273246
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

HAM’S F-12K Standard Formulation, HAM’S F-12K w/o Glutamine, HAM’S F-12K w/o Phenol Red, HAM’S F-12K w/o Sodium Bicarbonate, HAM’S F-12K w/o Sodium Pyruvate, HAM’S F-12K w/o Glucose, HAM’S F-12K w/o Lipoic Acid, HAM’S F-12K w/o Glutamine, w/o Phenol Red, HAM’S F-12K w/o Phenol Red, w/o Sodium Bicarbonate, HAM’S F-12K w/o Sodium Bicarbonate, w/o Sodium Pyruvate, HAM’S F-12K w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, HAM’S F-12K w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, HAM’S F-12K w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, HAM’S F-12K High Glucose (4.5 g/L), HAM’S F-12K Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K High Sodium Bicarbonate (3.7 g/L), HAM’S F-12K 15 mM HEPES (3.6 g/L), HAM’S F-12K 25 mM HEPES (5.9 g/L), HAM’S F-12K 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, HAM’S F-12K w/o Phenol Red, with 15 mM HEPES (3.6 g/L), HAM’S F-12K w/o Phenol Red, with 25 mM HEPES (5.9 g/L), HAM’S F-12K w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), HAM’S F-12K w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), HAM’S F-12K w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L) Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, with 25 mM HEPES (5.9 g/L) Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), HAM’S F-12K w/o Phenol Red, w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), HAM’S F-12K w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Ham’s F-12K Formulation

SDS

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