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Hybridoma Cryoprotective Media (HCM); DMSO Free

Hybridoma Cryoprotective Media (HCM); DMSO Free

(18 customer reviews)

PurMa™ Hybridoma Cryoprotective Media (HCM); DMSO Free is an excellent choice for cryopreservation of hybridoma cell lines.  PurMaTM HCM has great performance in preservation of fused myeloma and CHO cells (producing antibodies) as well as humanized Hybridomas. It contains a proprietary combination for the best hybridoma cryopreservation, survival, and recovery after thawing.

SKU: N/A Categories: Cryoprotective Media, Cryoprotective media DMSO-Free Tags: Antibody Producing Cells, Cryoprotective Media, DMSO Free, Hybridoma

Description

Background

PurMaTM Hybridoma Cryoprotective Media (HCM); DMSO Free is an excellent choice for cryopreservation of hybridoma cell lines. We strongly recommend applying DMSO free cryoprotective media only for cell lines with sensitivity to DMSO.  PurMaTM HCM has great performance in preservation of fused myeloma and CHO cells (producing antibodies) as well as humanized Hybridomas. It contains a proprietary combination for the best hybridoma cryopreservation, survival, and recovery after thawing.

DMSO benefits cryoprotection and cryoprotective agent in several ways

  1. Provides strong molecular stability.
  2. Besides, facilitates a high non-conductor constant.
  3. It contains the basic properties, salts solubility particularly for anions. Additionally contains a propensity to act as the acceptor atom in hydrogen bonding.
  4. Especially,  DMSO makes a  hydrogen bond with water which is 30% stronger than between two water molecules.
  5. The proprietary formulation of PurMaTM Mammalian Cryoprotective Media (MCM) allows the MCM to get inside the cells instantly and lower cell death because of penetration/freezing.

Although, DMSO with the penetrating ability, is a strong cryoprotective agent, there is however some limitations and disadvantages. For more details about the beneficial role of DMSO,  please see our other cryoprotective media with DMSO, such as:

limitations and disadvantages of DMSO

  1. Basically, it has a broad toxicity.
  2. Additionally, it modifies the expression of cells such an NK and T cells
  3. It alters the mRNA level of the de novo DNA methyltransferase DNMT3A. this therefore, accompanies by hyper- or hypo-methylation of many genetic loci.
  4. Moreover, it causes difficulties in scaling up DMSO-based cryoprotectants.
  5. It damages the mitochondria in certain cell culture models such as astrocytes.
  6. It negatively impacs cellular membrane/cytoskeleton structure integrity by interacting with proteins and dehydrating lipids.
  7. Besides, it Induces unwanted stem cell differentiation.
  8. Affects cellular epigenetic profile resulting in undesirable phenotypic disturbances. That is basically because of repeated sage of DMSO even at sub-toxic levels.
  9. Afterwards, it is necessary to remove DMSO by intensive washing. That in turns results in cell sensitivity to osmotic/mechanical stresses occurring. This is a costly- and time-consuming process during freezing and thawing process and moreover leads to a significant loss of cell number.

PurMaTM Hybridoma Cryoprotective Media (HCM); DMSO Free provides high post-thaw cell viability. Besides, it preserves morphology, proliferation as well as  the differentiation potentials of hybridoma cells.

Hybridoma Cryoprotective Media (HCM); DMSO Free Testing Table

Special Features

  • Ready-to-Use medium.
  • It does not contain DMSO or any other penetrating agents.
  • Each Lot of PurMaTM HCM goes through pathogens and sterility evaluations.
  • Additionally, we perform the following survival tests on each Lot of PurMaTM HCM:  Survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and finally,  for the rate of recovery by Alamar Blue test.
  • Moreover, PurMaTM HCM has been designed for the lowest cell loss during freezing and thawing.
  • PurMaTM HCM yields highest cell recovery, 64% survival and recovery rate after long term cryopreservation.
  • PurMaTM HCM gives faster recovery which results in less exposure to the toxins from the non-recovering cells.
  • Preserves morphological damage as the main recovering factor for cells.
  • Tested for pH, osmolality, sterility, and endotoxin.
  • PurMaTM HCM has been tested successfully for several antibody making cell lines including fused NS1 and CHO cells.
  • Suitable for clinical applications.

Guideline

for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines

References

  1. Differentiation of erythroleukemic cells in vitro: irreversible induction by dimethyl sulfoxide (DMSO). Preisler et al. 1975 Jun;85(3):537-46. doi: 10.1002/jcp.1040850305.
  2. Dimethyl sulfoxide-free cryopreservation solutions for hematopoietic stem cell grafts. Kaushal et al. 2022 Mar;24(3):272-281. doi: 10.1016/j.jcyt.2021.09.002. Epub 2021 Oct 12.

 

Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Size

1 x 10 ml, 1 x 100 ml

18 reviews for Hybridoma Cryoprotective Media (HCM); DMSO Free

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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