Iscove’s Modified Dulbecco’s Medium (IMDM)
Although, Iscove’s Modified Dulbecco’s Medium (IMDM) is specialized for rapidly proliferating, cell lines, it certainly quite suitable for slow growing cells as well. Moreover, this medium is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate , therefore makes it an excellent choice for adhesive cells.
Description
Iscove’s Modified Dulbecco’s Medium (IMDM)
Background
Iscove’s Modified Dulbecco’s Medium (IMDM) is specialized for rapidly proliferating, high-density cell lines. Therefore, it is excellent for Jurkat, Hek, COS-7, and macrophage cells. IMDM is a modified and more advanced version of DMEM. Additionally, it includes selenium as well as additional amino acids and vitamins. it is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate, therefore it makes it excellent for adhesive cells.
Ingredients
It contains:
- High Buffering Capacity with High NaHCO3 as well as High HEPES
- Sodium Bicarbonate (3.02 g/L)
- 5.9 g/L HEPES
- High glucose 4.5g/L
- Non-Essential Amino Acids
- 110 mg/L Sodium pyruvate (1.0 mM)
- 15 mg/L Phenol Red
- 0.584 g/L L-glutamine
Formulation
Complete formulation is available on our website as well as here : IMDM Formulation
References
- Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | IMDM Standard Formulation, IMDM Standard Formulation, ATCC Formulation, IMDM w/o Glutamine, IMDM w/o Phenol Red, IMDM w/o Sodium Bicarbonate, IMDM w/o Sodium Pyruvate, IMDM w/o Glucose, IMDM w/o HEPES, IMDM w/o Glutamine, w/o Phenol Red, IMDM w/o Phenol Red, w/o Sodium Bicarbonate, IMDM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, IMDM w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, IMDM Low Glucose (1.5 g/L), IMDM 15 mM HEPES (3.6 g/L), IMDM 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), IMDM w/o Sodium Pyruvate, w/o Phenol Red, IMDM w/o Sodium Pyruvate, w/o HEPES, IMDM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), IMDM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), IMDM w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), IMDM w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]Formulation
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