Kaighn’s Modification of Ham’s F-12-K Medium
Kaighn’s Modification of Ham’s F-12-K Medium is one of the widely used media. PurMa Biologics manufactures 96 types of this media. Moreover, (Ham’s F-12K) contains components not found in older media, such asputrescine thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate
Description
Kaighn’s Modification of Ham’s F-12-K Medium
Background
Kaighn’s Modification of Ham’s F-12-K Medium is a modified version of Ham’s F-12 medium. originally, Ham’s F-12K (Kaighn’s) was used for primary cells such as human oocytes and hepatocytes, as well murine and chicken liver cells in a lower serum environment. but, however, recently this media is used for a much larger variety of cells.
Moreover, Ham’s F-12K (Kaighn’s) Medium contains a variety of components not found in older media, such as putrescine thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate.
Ingredients:
F-12K Medium also contains:
– Low level of glucose (1.26 g/ liter)
– Sodium bicarbonate (2.5 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed).
– Additionally, PurMa Biologics also offers F-12K Medium with different level of sodium bicarbonate : 1.5 g/L (Cat # P3S057108) as well as, 3.7g/L (Cat # P3S032108)
– 2 mM L-glutamine (292 mg/L)’
– A higher concentration of amino acids, vitamins, inositol and choline are present in this medium.
Why PurMa Biologics? Why we stand behind our Cell Culture Media:
Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science. Also, we utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.
We routinely manufacture over 1500 media, which is the most comprehensive collection of cell and tissue culture media. Considering the highest quality, our prices are extremely reasonable.
Application of Ham’s F-12K:
This media has elements that prevent clumping, therefore, it is ideal for culturing primary as well as commercial cell lines, for instance normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells.
Formulation:
For complete formulation click here: Ham’s F-12K Formulation
References:
- Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss et al. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
- Human recombinant insulin-like growth factor I. I. Development of a serum-free medium for clonal density assay of growth factors using BALB/c 3T3 mouse embryo fibroblasts. Riss et al. In Vitro Cell Dev Biol. 1988 Nov;24(11):1099-106. doi: 10.1007/BF02620811.
- Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium. Fong et al. Diabetes. 1981 Dec;30(12):1022-8. doi: 10.2337/diab.30.12.1022. PMID: 6273246
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | HAM’S F-12K Standard Formulation, HAM’S F-12K Standard Formulation, ATCC Formulation, HAM’S F-12K w/o Glutamine, HAM’S F-12K w/o Phenol Red, HAM’S F-12K w/o Sodium Bicarbonate, HAM’S F-12K w/o Sodium Pyruvate, HAM’S F-12K w/o Glucose, HAM’S F-12K w/o Lipoic Acid, HAM’S F-12K w/o Glutamine, w/o Phenol Red, HAM’S F-12K w/o Phenol Red, w/o Sodium Bicarbonate, HAM’S F-12K w/o Sodium Bicarbonate, w/o Sodium Pyruvate, HAM’S F-12K w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, HAM’S F-12K High Glucose (4.5 g/L), HAM’S F-12K Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K High Sodium Bicarbonate (3.7 g/L), HAM’S F-12K 15 mM HEPES (3.6 g/L), HAM’S F-12K 25 mM HEPES (5.9 g/L), HAM’S F-12K 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, HAM’S F-12K w/o Phenol Red, with 15 mM HEPES (3.6 g/L), HAM’S F-12K w/o Phenol Red, with 25 mM HEPES (5.9 g/L), HAM’S F-12K w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), HAM’S F-12K w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]Formulation
SDS
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