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Lipid Depleted Fetal Horse Serum

Lipid-Depleted Fetal Horse Serum (LD-FHS)

(18 customer reviews)

Since the serum usually presents in cell culture studies, lipid metabolism makes studying the role of serum in cultured cells very challenging.  That is because serum contains a wide range of undefined lipid species. PurMa™ Lipid-Depleted Fetal Horse Serum (LD-FHS) performs the highest level of lipid depletion in serum.

SKU: N/A Categories: Fetal Bovine Serum, Horse Serums Tags: Fetal Horse serum, Lipid-Depleted

Description

Background

Since the serum usually  presents in cell culture studies,  the lipid metabolism makes studying the role of serum in cultured cells very challenging.  That is because serum contains a wide range of undefined lipid species. PurMa™ Lipid-Depleted Fetal Horse Serum (LD-FHS) performs as the highest level of lipid depletion of serum . Lipid-Depleted Fetal Horse Serum (LD-FHS) performs crucial roles in:

  1. Where the normal concentration of lipids may interfere with the results.
  2.  It Pays  also crucial for studies directly involving lipid metabolism. Because the composition of serum can have a profound impact on important cellular checkpoints.
  3. Moreover, LD-FHS performs in investigations about the role of lipids in their corresponding intracellular pathways.
  4. Also, it acts to generate  the media with defined lipid species.

PurMa Biologics uses a cutting-edge methodology to remove lipids from serum without eliminating its ability to support the proliferation of cells in culture.

Our proprietary method includes

  1. Applying isopropyl ether and butanol with a special ratio.
  2. PurMa Biologics has spent many years determining the needed density of ether and butanol as well as the ratio of these two solvents in this process to gain the best result.
  3. 4X washed each 3h hours with isopropyl ether-butanol with decreasing amount of butanol in every washing, in a way that the last two wash is carried out without butanol.
  4. Dialyzing with a 3500 Dalton dialysis tube for 4 days with low salts physiological buffer at physiological pH.
  5. To prepare 500 ml Lipid-Depleted Fetal Horse Serum (LD-FHS), 10 changes of 10 liters of buffer is needed.
  6. The dialysis will be terminated when the buffers show no isopropanol using GC–MS.
  7. The serum  goes through a vigorous analysis for pathogens as well as growth and MTT (proliferation ability) using 2 cell lines. For complete details, please see below: quality control as well as “Analysis Tab”).
  8. The resulting high-quality serum lacks most of lipids and can still be used to support the growth and maintenance of many cell lines in culture.
  9. USE NOT LESS THAN 15% SERUM IN YOUR MEDIA

Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums

Special Features

  • PurMa™Lipid-Depleted Fetal Horse Serum (LD-FHS) is of USA origin.
  • We sterilize the serums with gamma irradiation.
  • PurMa™ LD-FHS has been heat inactivated.
  • PurMa™Lipid-Depleted Fetal Horse Serum (LD-FHS)has been depleted of lipids and dialyzed using the 3500 Dalton dialysis tube.
  • Is free of lipids, phospholipids, and a long chain of fatty acids
  • Has been vigorously analyzed with the certificate of analysis.
  • Free of Mycoplasma, IgG, steroids and hormones, and other pathogens.
  • Acceptable level of Endotoxin hemoglobin (20 mg/DL) and endotoxin (10 mg/DL).

References

  1. Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
  2. Changes in serum influence the fatty acid composition of established cell lines. Stoll et al. In Vitro. 1984 Sep;20(9):732-8.

 

Methodology Unit measured Accepted level Result
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) NA Not Detected Not Detected
pH N/A measured 6.58
Osmolality (USP 785)     mOsm/KgH2 mOsm/KgH20 280-340 288
Hemoglobin (Fleming & Woolf) mg/dL <25 12.9
Endotoxin (USP 85) EU/mL <20 <0.100
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) NA Not Detected Not Detected
IgG Elliza Measured 0.19 ug/ml
Albumin Eliza Measured ≤ 4.6 g/dL
Virus Testing
Horse Rhinopneumonitis/Equine Herpesvirus (EHV) NA Not Detected Not Detected
Horse Equine Encephalomyelitis NA Not Detected Not Detected
Horse Equine Infectious Anemia Virus (EIA) NA Not Detected Not Detected
Horse West Nile Virus NA Not Detected Not Detected
Rabies NA Not Detected Not Detected
Reovirus NA Not Detected Not Detected
Cytopathogenic Agents (IBR) NA Not Detected Not Detected
Hemadsorbing Agents (PI3) NA Not Detected Not Detected
Bluetongue NA Not Detected Not Detected

 

Additional information

Size

1 x 50 ml, 1 x 500 ml

18 reviews for Lipid-Depleted Fetal Horse Serum (LD-FHS)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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