Description

MEM Alpha Modification in Earle’s Buffer (MEM-α)

Background

MEM Alpha Modification in Earle’s Buffer (MEM-α) is certainly a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.

MEM -α  contains

• Non-essential amino acids.
• Sodium pyruvate.
• Lipoic acid.
• Vitamin B12.
• Biotin and,
• Ascorbic acid.
• Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.
• Moreover, PurMa^TM MEM -α media in Earle’s buffer includes Earles’ buffer

The composition of Earle’s buffer, it contains:

1. Calcium chloride
2. Magnesium sulfate
3. Potassium chloride
4. Sodium bicarbonate
5. Sodium chloride
6. Sodium Phosphate, monobasic

Additionally, MEM Alpha Modification in Earle’s Buffer (MEM-α)

it contains:

• Low level of glucose (1.00 g/ liter)
• More importantly, Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
• 2 mM L-glutamine (292 mg/L)’
• Also, PurMa Biologics manufactures 118 types of MEM-α

Application

This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes as well as human melanoma cells. Furthermore, Alpha modification of Minimum Essential Medium Eagle has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast.

Why PurMa Biologics? Why we stand behind our cell culture media.

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, we utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Formulation

Complete formulation is in the ” Formulation tab” as well as here: MEM-alpha Formulation in Earle’s Buffer

References

1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay et al.  Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
2. Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)