Description

MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS)

Background

MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside, and the formulation is available below. depending on cell types, laboratories use MEM-α-N for growing mammalian cell for a variety of suspension as well as adherent cells. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.

Ingredients

MEM Alpha Modification with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a modification of MEM.

MEM-α-N-EBSS contains:

• Non-essential amino acids.
• Sodium pyruvate.
• Lipoic acid.
• Vitamin B12.
• Biotin and,
• Ascorbic acid.

PurMa™ MEM-α-N-EBSS includes Earles’ Buffer

As the composition of Earls buffer, it contains:

1. Calcium chloride
2. Magnesium sulfate
3. Potassium chloride
4. Sodium bicarbonate
5. Sodium chloride
6. Sodium Phosphate, monobasic

PurMa™ MEM -α-N-EBSS media also contains:

• Low level of glucose (1.00 g/ liter)
• Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
• 2 mM L-glutamine (292 mg/L)’
• PurMa Biologics manufactures 121 types of MEM -α-N-E

The nucleoside modifies this media  from MEM-α

it contains:

• Ribonucleosides
• Deoxyribonucleosides

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Application

This media can also be used for keratinocytes, primary rat astrocytes, as well as human melanoma cells. MEM-α-N-EBSS has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast. Additionally, PurMa™ MEM -α-N-EBSS facilitates the growth and maintenance of primary osteoblast cultures. furthermore, this media performs well for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

Formulation

Click here for complete formulation as well as in ” Formulation Tab”: MEM alpha with Nucleoside Formulation

References

1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay, L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
2. Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction.  Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
3. Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun et al. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.