Description

MES Stock Buffer Solution

Background

MES Stock Buffer Solution is one of the commonly used buffering agents in cell culture, biology, and biochemistry. It, however, does not form complexes with most of the metals used in environmental and biological studies. It easily dissolves in water and has minimum lipid solubility, making it impermeable to membranes. Moreover, MES buffer induces autophagy in cells. We also produce this widely used buffer in various pH formats.

Grades of MES Stock Buffer Solution

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

we finally sterilize the buffers by autoclaving the solution and packing  in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2

The Endotoxin Removing Procedure from Buffer

PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer  through the PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B   to an endotoxin-specific absorbent. we finally  eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

Features

• Cell Culture Suited as well as molecular biology suited.
• Provides a buffer solution to maintain a medium in physiological pH (7.2-7.6).
• PurMa Biologics manufactures this buffer free of  Mg²⁺ and Ca ²⁺
• Free of RNAse /DNase
• Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
• We rigorously test for contamination of endotoxin and mycoplasma.
• PurMa Biologics manufactures this buffer in several pH formats.
• We sterilize it by 0.2µm filter.
• PurMa Biologics pack all the buffers under N₂, so the pH does not change over time before opening the bottle.

Application

It plays a significant part in preparation of Murashige and Skoog growth medium for the growth of cannabis in cell culture. In molecular biology, MES is commonly used in the conjugation of hybridization probes to beads before PCR amplification.

References

1. Hydrogen ion buffers for biological research. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RM. Biochemistry. 1966 Feb;5(2):467-77. doi: 10.1021/bi00866a011. PMID: 5942950.
2. Noncomplexing Tertiary Amines as “Better” Buffers Covering the Range of pH 3-11. Temperature Dependence of Their Acid Dissociation Constants. Kandegedara A, Rorabacher DB. Anal Chem. 1999 Aug 1;71(15):3140-4. doi: 10.1021/ac9902594. PMID: 21662904.