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Mycoplasma Removal Spray

Mycoplasma Removal Spray

(18 customer reviews)

PurMa™ Mycospray is the most sophisticated Mycoplasma removal agent currently available in the market. Currently, An estimated 25% of U.S. laboratory cell cultures are contaminated with various species of mycoplasma.

SKU: N/A Category: Mycoplasma Detection and Elimination Reagents Tags: Mycoplasma Removal, Mycoplasma Spary

Description

Background

PurMa™ Mycospray is the most sophisticated Mycoplasma removal spray currently available in the market. Currently, an estimated 25% of U.S. laboratory cell cultures are contaminated with various species of mycoplasma.

Source of Mycoplasma to your laboratory

  1. The lack of HEPA filter and positive pressure. Without the mentioned condition, there is certainly a flow of mycoplasma in the lab.
  2. Moreover, lab personnel is the second main source of mycoplasma contamination.

So, entering mycoplasma to your tissue culture room is inevitable. As a result,  the tissue culture hood, CO2 incubator, benches, pipettors and the gloves you wear need to be routinely treated by a mycoplasma disinfectant.

PurMa™ Mycoplasma Removal Spray Special Features

  • PurMa™ Mycospray is formulated to destroy the mycoplasma nuclear composition and will not leave any mycoplasma if it is used as instructed below.
  • Moreover, PurMa™ Mycospray is safe for skin and does not create any vapor to inhale at room temperature.
  • PurMa™ Mycospray destroys the following species with 100% certainty: A. laidlawii, M. arginine, M. boris, M. fermentans M. hominis, M. hyorhinis and M. orale.

Instruction to use

  1. Because of the massive flow of mycoplasma to your tissue culture room, PurMa™ Mycospray needs to be used routinely at least three times per week.
  2. After completing work in tissue culture lab, routinely, wipe inside the hood and all the surfaces including the microscope area where you view your cells as well as the pipets. This is however, applies to other instruments which you do not keep under the UV light.
  3. Apply PurMa™ Mycospray , DO NOT dry it. It is therefore, crucial to leave the surface wet as it takes 5 minutes to complete the destruction of mycoplasma nuclei membrane.
  4. On the following day clean the mentioned surfaces with 70% alcohols to remove the residues of the dead mycoplasmas.
  5. Wear mask at all times inside the tissue culture lab as breathing is one of the main sources of several mycoplasma including m. Fermentans m. Hominis, m. Hyorhinis and m. Orale
  6. Wash your gloves with PurMatm Mycospray before any cell culture work

References

  1. Bioprocess: Robustness with Respect to Mycoplasma Species. Faison et al. PDA J Pharm Sci Technol. 2020 Mar-Apr;74(2):201-212. Epub 2019 Sep 13.
  2. Mycoplasma removal: simple curative methods for viral supernatants. Baronti Cet al. J Virol Methods. 2013 Feb;187(2):234-7.. Epub 2012 Oct 29.
Parameter Specification
Appearance Clear  liquid
pH 8.6 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability Suitable for mammalian cell culture

NOT to be in contact with Cells
Indicator NA
Sterility Tested Sterile filtered using 0.1 µm filter
Form Liquid
Shipping Condition Ambient

 

Additional information

Size

500 ml spray (1X), 500 ml (10X), 1 Liter (10X)

18 reviews for Mycoplasma Removal Spray

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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