PurMa™ Roswell Park Memorial Institute 1640 (RPMI-1640)
PurMa™ RPMI-1640 cell culture medium is a high‑performance formulation optimized for mammalian cells, including tumor and suspension lines. Manufactured with 99% pure amino acids and backed by 30 years of cell culture expertise. PurMa™ RPMI-1640 cell culture medium delivers exceptional batch‑to‑batch consistency, reliable growth, and excellent reproducibility at a very competitive price.
Description
PurMa™ RPMI-1640 Cell Culture Medium – High‑Consistency Mammalian Cell Culture Media
Product Description
PurMa™ RPMI-1640 cell culture medium is a premium formulation designed for reliable growth of a wide range of mammalian cell lines. Originally developed in 1967 at Roswell Park Memorial Institute, RPMI-1640 is now a standard medium for suspension and tumor cells. Our optimized PurMa™ RPMI-1640 cell culture medium builds on this classic formulation to support robust proliferation and high experimental reproducibility. RPMI 1640 originally developed to culture human ( recombinant human flt3 ligand protein)
PurMa™ RPMI-1640 cell culture medium contains elevated phosphate levels that support tumor cells relying on glycolysis as their main energy source. This makes it especially suitable for cell aggregates and cancer research applications. The medium has a higher pH of approximately 7.6, and includes biotin, vitamin B12, and PABA, along with high concentrations of inositol and choline. These components help maintain healthy cell metabolism and stable long‑term cultures.
We manufacture PurMa™ RPMI-1640 cell culture medium using 99% pure amino acids and strict quality controls. Our production is based on more than 30 years of experience in cutting‑edge cell and tissue culture science. PurMa Biologics routinely produces over 1500 media formulations, ensuring deep expertise in nutrient balance, sterility, and performance. This high level of purity and process control translates into superior consistency between batches and more comparable results between laboratories.
PurMa™ RPMI-1640 cell culture medium is successfully used for culturing human normal and neoplastic leukocytes, HeLa, Jurkat, MCF‑7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells. Whether you work in oncology, immunology, neuroscience, or bioproduction, this medium provides a reliable platform for routine culture, assay development, and advanced research.
Considering its quality and performance, PurMa™ RPMI-1640 cell culture medium is offered at an extremely reasonable price point. It is an excellent choice for laboratories that need consistent, high‑quality cell culture media without compromising their budget.
FAQs
Q1. What is PurMa™ RPMI-1640 cell culture medium used for?
PurMa RPMI-1640 cell culture medium is used for culturing human normal and neoplastic leukocytes, as well as many common cell lines. Typical applications include HeLa, Jurkat, MCF‑7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells. It is widely used in cancer research, immunology, and general mammalian cell biology.
Q2. How is PurMa™ RPMI-1640 different from other cell culture media?
PurMa RPMI-1640 cell culture medium has a higher pH of around 8 and contains elevated phosphate levels that support glycolysis‑dependent tumor cells. It also includes biotin, vitamin B12, and PABA, plus high concentrations of inositol and choline. These features distinguish it from other standard media and make it especially suitable for tumor and suspension cultures.
Q3. Why should I choose PurMa RPMI-1640 over standard RPMI-1640?
PurMa™ RPMI-1640 cell culture medium is manufactured with 99% pure amino acids and strict quality controls. Our 30 years of experience and production of more than 1500 media formulations ensure excellent batch‑to‑batch consistency. This leads to more reliable data, closer alignment between experiments, and better comparability with results from other laboratories.
Q4. Which cell types grow best in PurMa™ RPMI-1640 cell culture medium?
PurMa RPMI-1640 cell culture medium is ideal for leukocytes, hybridomas, and many tumor and suspension cell lines. It performs particularly well with HeLa, Jurkat, MCF‑7, PC12, PBMC, astrocytes, carcinomas, and bone marrow cultures. Always confirm compatibility with your specific cell line and adapt supplements as needed.
Q5. Does PurMa™ RPMI-1640 contain amino acids, vitamins, and other nutrients?
Yes. PurMa RPMI-1640 cell culture medium contains a balanced mixture of amino acids, vitamins, and inorganic salts. In addition to its high phosphate level, it includes biotin, vitamin B12, PABA, inositol, and choline in high concentrations. These components help support stable growth and maintain healthy cell metabolism.
Q6. Where can I find the full formulation of PurMa™ RPMI-1640 cell culture medium?
The complete formulation of PurMa RPMI-1640 cell culture medium is available on our website. Please refer to the RPMI-1640 Formulation section on this product page or follow the provided formulation link to see the full list of components and their concentrations.
Formulation
Formulation: Complete formulation is here: RPMI-1640 Formulation
The Standard formulation pf PurMa™ RPMI-1640 is identical to Thermofisher, Gibco RPMI 1640 medium and slightly different than ATCC formulation. PurMa Biologics, however manufactures the ATCC RPMI1640 routinely.
References
- Culture of normal human leukocytes. Moore et al. 1967 Feb 20;199(8):519-24. PMID: 4960081
- Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. Dunne et al. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | RPMI-1640 Standard Formulation, RPMI-1640, Standard Formulation (ATCC formulation), RPMI-1640 w/o Glutamine, RPMI-1640 w/o Phenol Red, RPMI-1640 w/o Sodium Bicarbonate, RPMI-1640 w/o Glucose, RPMI-1640 w/o Glutamine, w/o Phenol Red, RPMI-1640 w/o Phenol Red, w/o Sodium Bicarbonate, RPMI-1640 w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, RPMI-1640 With Low Glucose (1 g/L), RPMI-1640 With High Sodium Bicarbonate (3.7 g/L), RPMI-1640 With 15 mM HEPES (3.6 g/L), RPMI-1640 With 25 mM HEPES (5.9 g/L), RPMI-1640 With 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (3.7 g/L), RPMI-1640 With 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (3.7 g/L, RPMI-1640 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), RPMI-1640 w/o Phenol Red, with 25 mM HEPES (5.9 g/L), RPMI-1640 w/o Phenol Red, High Sodium Bicarbonate (3.7 g/L |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
43 reviews for PurMa™ Roswell Park Memorial Institute 1640 (RPMI-1640)
Only logged in customers who have purchased this product may leave a review.
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azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 21-Feb-2025 15:30:00
Ease of placing the order: ‘not given’
Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 24-Feb-2025 13:50:14
Ease of placing the order: ‘not given’
lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 27-Feb-2025 17:42:44
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 16:31:44
Ease of placing the order: ‘not given’
michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 17:29:13
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 10-Mar-2025 14:32:38
Ease of placing the order: ‘not given’
jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5
hyeungk1968@gmail.comthe –
Review: I used Lipid Free horse serum of PurMa Biologics.
The quality is great!
Have you purchased: Yes
Time of submission: 17-Mar-2025 12:09:58
Ease of placing the order: 5/5
jaya-krishnan@omrf.org –
Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
Have you purchased: Yes
Time of submission: 17-Mar-2025 14:22:58
Ease of placing the order: 4/5
tayyab-afzal@omrf.org –
Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
Have you purchased: Yes
Time of submission: 18-Mar-2025 10:01:50
Ease of placing the order: 5/5
Fitzkr@gmail.com –
Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
Always great quality, fast processing and shipping.
We are totally satisfied.
Have you purchased: Yes
Time of submission: 22-Mar-2025 10:47:00
Ease of placing the order: 5/5
vmadka@ouhsc.edu –
Review:
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:02:40
Ease of placing the order: 4/5
Anupam-Mandal@ouhsc.edu –
Review: Excellent
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:05:43
Ease of placing the order: 5/5
rohan-varshney@ouhsc.edu –
Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:06:38
Ease of placing the order: 5/5
Surendra-Shukla@ouhsc.edu –
Review: Very fast, efficient, and economic service. Great for new PIs like me.
Have you purchased: Yes
Time of submission: 25-Apr-2025 14:00:04
Ease of placing the order: 5/5
vmadka@ouhsc.edu –
Review:
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:02:40
Ease of placing the order: 4/5
Anupam-Mandal@ouhsc.edu –
Review: Excellent
Have you purchased: Yes
Time of submission: 24-Mar-2025 15:05:43
Ease of placing the order: 5/5
anil-singh@ouhsc.edu –
Review: NA
Have you purchased: Yes
Time of submission: 25-Mar-2025 10:11:14
Ease of placing the order: 5/5
Surendra-Shukla@ouhsc.edu –
Review: Best quality products, best price.
Have you purchased: Yes
Time of submission: 18-Apr-2025 09:51:03
Ease of placing the order: 5/5
madhusmita-rout@ouhsc.edu –
Review:
Have you purchased: Yes
Time of submission: 21-Apr-2025 11:15:46
Ease of placing the order: 5/5
alexholloway@mesogen.com –
Review: This serum appears to be a high quality product and worked well for our needs. Purma has an extensive catalog for cell culturing supplies which we are glad we came across.
Have you purchased: It is my first Purchase
Time of submission: 24-Apr-2025 16:54:29
Ease of placing the order: 5/5
jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5
lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
hyeungk1968@gmail.comthe –
Review: I used Lipid Free horse serum of PurMa Biologics.
The quality is great!
Have you purchased: Yes
Time of submission: 17-Mar-2025 12:09:58
Ease of placing the order: 5/5
jaya-krishnan@omrf.org –
Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
Have you purchased: Yes
Time of submission: 17-Mar-2025 14:22:58
Ease of placing the order: 4/5
michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
tayyab-afzal@omrf.org –
Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
Have you purchased: Yes
Time of submission: 18-Mar-2025 10:01:50
Ease of placing the order: 5/5
Fitzkr@gmail.com –
Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
Always great quality, fast processing and shipping.
We are totally satisfied.
Have you purchased: Yes
Time of submission: 22-Mar-2025 10:47:00
Ease of placing the order: 5/5
Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Ravi-Gor@ouhsc.edu –
Review: I recently had the pleasure of interacting with Dr. Seif Azadi, and I want to express my gratitude for his expertise and timely assistance. His knowledge and dedication were instrumental in addressing our lab’s needs efficiently.
Have you purchased: It is my first Purchase
Time of submission: 25-Jun-2025 12:00:09
Ease of placing the order: 5/5
xiangpeng-li@ou.edu –
Review: PurMa helped us with the customized medium. They are professional and friendly. I appreciate their great work.
Have you purchased: Yes
Time of submission: 14-Aug-2025 12:59:31
Ease of placing the order: 5/5
jianhua.song@appliedstemcell.com –
Review: it is reliable vendor!
Have you purchased: Yes
Time of submission: 07-Oct-2025 17:14:12
Ease of placing the order: 5/5
Zhenghui.Liu@appliedstemcell.com –
Review: We always buy our agarose from PurMa and have never been disappointed.
Have you purchased: Yes
Time of submission: 08-Oct-2025 11:17:53
Ease of placing the order: 5/5
ravi-gor@ou.edu –
Review: I recently had the pleasure of interacting with Dr. Seif Azadi from Purma Biologics, and I cannot thank him enough for his expertise and prompt assistance. His deep knowledge and dedication were instrumental in meeting our laboratory’s needs with remarkable efficiency.
We ordered FBS, DMEM, and Trypsin from Purma Biologics, and I’m thrilled to report that all the products have performed exceptionally well. They consistently deliver reproducible results, significantly enhancing the reliability of our experiments.
Thank you, Team Purma Biologics, for your good products and support!
Have you purchased: It is my first Purchase
Time of submission: 23-Oct-2025 17:30:25
Ease of placing the order: 5/5
info@takbiotechs.com –
Review: We are extremely satisfied with the quality and consistency of PURMA Biologics products. Both the FBS and cell culture media have delivered excellent performance in our applications, with reliable batch-to-batch consistency. Highly recommended for research and bioprocessing use.
Have you purchased: Yes
Time of submission: 11-Apr-2026 14:49:27
Ease of placing the order: 5/5
aslan.batur@aytakbiotech.com –
Review: We’ve had a great experience with PURMA Biologics products. In particular, their FBS has shown excellent consistency and performance, and their DMEM media has worked very well in our cell culture applications. Overall, very reliable quality.
Have you purchased: Yes
Time of submission: 11-Apr-2026 14:57:39
Ease of placing the order: 5/5
maya.hart@takbiomed.com –
Review: It’s been a pleasure working with PURMA Biologics. Their service is efficient, delivery is fast, and the quality of their products has met our expectations consistently. Very smooth collaboration overall.
Have you purchased: Yes
Time of submission: 11-Apr-2026 15:06:47
Ease of placing the order: 5/5
hajipour@etsu.edu –
Review: PurMa products are of excellent quality and highly reliable.
Have you purchased: Yes
Time of submission: 11-Apr-2026 16:23:59
Ease of placing the order: 5/5