Description

EDTA Buffer

Background

PurMa Biologics manufactures various grades of PurMa EDTA Buffer Solution NaEDTA•2HO using different grades of water ( see below). Moreover, EDTA solution is suitable for biochemistry or molecular biology applications requiring a chelator of divalent metal ions. Additionally, we adjust the pH to 8.0 with sodium hydroxide.

Grades of EDTA Buffer

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

we finally sterilize the buffers by autoclaving the solution and packing  in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2

The Endotoxin Removing Procedure from Buffer

PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer  through the PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B   to an endotoxin-specific absorbent. we finally  eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

Features

• Cell Culture Suited.
• provides a  cost-effective and easy to use buffer.
• Suitable for a variety of biochemical applications.
• EDTA  chelates  heavy metals such as Mg²⁺ and Ca ²⁺.
• PurMa Biologics manufactures EDTA in Mg²⁺ and Ca ²⁺-free water.
• Free of RNAse /DNase
• Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
• It is rigorously tested for contamination of endotoxin and mycoplasma.
• Is manufactured in two pH formats: pH: 8.00 ± 0.01and 8.5 ± 0.01 at 25°C.
• Sterile filtered by 0.22µm membrane (in case of endotoxin depleted HBSS by 0.1µm)
• It is packed under N_2; therefore, the pH won’t change over time before opening the bottle.

Formulation

For complete formulation, click here:EDTA Formulation

References

1. Purification of two endonucleases from potato tubers. BJOERK W. Biochim Biophys Acta. 1965 Apr 19;95:652-62. PMID: 14324816.
2. Chelating agents. Bergan T, Klaveness J, Aasen AJ. Chemotherapy. 2001 Jan-Feb;47(1):10-4. doi: 10.1159/000048495. PMID: 11125227.