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Roswell Park Memorial Institute 1640 (RPMI-1640)

Roswell Park Memorial Institute 1640 (RPMI-1640)

(18 customer reviews)

Roswell Park Memorial Institute 1640 (RPMI-1640)  are being  used for culturing human normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, as well as hybridoma cells.

SKU: N/A Categories: Cell Culture Media, Roswell-Park Memorial Institute 1640 Tags: Cell Culture, Phenol Red, RPMI, RPMI 1640, Standard

Description

Background:

Roswell Park Memorial Institute 1640 (RPMI-1640) was originally developed in 1967 by Moore et. Al at Roswell Park Memorial Institute. Therefore, it has the acronym RPMI-1640. Moreover, this medium contains a great deal of phosphate which is an essential element for tumor cells due to using glycolysis as a main source of energy in cell aggregates which is one of the most useful strategies for tumor cells survival.

Why PurMa Biologics? Why we stand behind our cell culture media:

Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science. Additionally, we routinely manufacture over 1500 media . Moreover, We utilize %99 pure amino acids. This significantly increases the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues. Considering the highest quality, our prices are extremely reasonable.

Application:

RPMI-1640 medium are being successfully used for culturing human normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells. The major difference between RPMI1640 and other media besides higher PH (8) is, it contains biotin, vitamin B12, and PABA. In addition to alterations in the amounts of amino acids and vitamins, inositol, and choline are present in very high concentrations.

Formulation:

Formulation: Complete formulation is here: RPMI-1640 Formulation

References:

  1. Culture of normal human leukocytes. Moore et al. 1967 Feb 20;199(8):519-24. PMID: 4960081
  2. Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. Dunne et al. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.
  3. Long-term culture of human leukaemic leukocytes. Pössnerová et al. 1970;17(5):513-23. PMID: 5274436

 

Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

RPMI-1640 Standard Formulation, RPMI-1640, Standard Formulation (ATCC formulation), RPMI-1640 w/o Glutamine, RPMI-1640 w/o Phenol Red, RPMI-1640 w/o Sodium Bicarbonate, RPMI-1640 w/o Glucose, RPMI-1640 w/o Glutamine, w/o Phenol Red, RPMI-1640 w/o Phenol Red, w/o Sodium Bicarbonate, RPMI-1640 w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, RPMI-1640 With Low Glucose (1 g/L), RPMI-1640 With High Sodium Bicarbonate (3.7 g/L), RPMI-1640 With 15 mM HEPES (3.6 g/L), RPMI-1640 With 25 mM HEPES (5.9 g/L), RPMI-1640 With 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (3.7 g/L), RPMI-1640 With 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (3.7 g/L, RPMI-1640 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), RPMI-1640 w/o Phenol Red, with 25 mM HEPES (5.9 g/L), RPMI-1640 w/o Phenol Red, High Sodium Bicarbonate (3.7 g/L
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

18 reviews for Roswell Park Memorial Institute 1640 (RPMI-1640)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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