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Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)

Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)

(18 customer reviews)

Endotoxin-Depleted Fetal Bovine Serum (ED-FBS) is highly evaluated for endotoxin.  Moreover, Following triple filtering with 0.1µm,  the residual of endotoxin were depleted using PurMaTM Endotoxin Elimination Column (Cat# P6H1216251)

SKU: N/A Categories: Bovine Serums, Fetal Bovine Serum Tags: Endotoxin Depleted, Fetal Bovine Serum

Description

Background

PurMa Biologics manufactures seven types of bovine serum, three of which however, considered as premium fetal bovine serums, each specialized for certain requirements in tissue culture. Moreover,  We highly evaluate Endotoxin-Depleted Fetal Bovine Serum (ED-FBS) for endotoxin and after triple filtering with 0.1µm,  the residual of endo toxin were depleted using PurMaTM Endotoxin Elimination Column (Cat# P6H1216251) ( see below).

PurMaTM Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)

  • PurMaTM Endotoxin Depleted Fetal Bovine Serum (EDP-FBS) is of USA origin.
  • Additionally, has gone through  an endotoxin depletion procedure.
  • Has gone through  gamma irradiation as well as heat inactivation process.
  • Lastly, we vigorously analyze various aspect and each LOT possess a  certificate of analysis.

Endotoxin Removal Procedure

  1. PurMa Biologics processes PurMaTM ED-FBS from PurMaTM Premium Fetal Bovine Serum (P-FBS).  It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.
  2. The process of removing endotoxin from FBS  routinely starts with passing the premium FBS through the PurMaTM Endotoxin Elimination Column (Cat# P6H1216251) with the rate of 0.1 ml/ minute.
  3. The unbound fractions pass through 0.1µm filters  for three times. The mentioned procedure eliminates the residual of endotoxin as well.
  4. We conjugate  Sepharose 6B in our proprietary column covalently to powerful endotoxin-specific absorbent.

Analysis

PurMa biologics routinely performs pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums

References

  1. Biochemical and morphological studies of steady state and lipopolysaccaride treated bovine articular cartilage explant cultures. Tian et al. Connect Tissue Res. 1989;19(2-4):195-218. doi: 10.3109/03008208909043897. PMID:
  2. The preparation of antigenic hapten-carrier conjugates: a survey. Erlanger BF. Methods Enzymol. 1980;70(A):85-104. doi: 10.1016/s0076-6879(80)70043-5.
Methodology Unit measured Accepted level Result
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) NA Not Detected Not Detected
pH N/A measured 6.58
Osmolality (USP 785)     mOsm/KgH2 mOsm/KgH20 280-340 288
Hemoglobin (Fleming & Woolf) mg/dL <25 12.9
Endotoxin (USP 85) EU/mL Not Detected Not Detected
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) NA Not Detected Not Detected
IgG Elliza Measured 0.19 ug/ml
Albumin Eliza Measured ≤ 4.6 g/dL
Virus Testing (9 CFR 113.53c)
Bovine Adenovirus NA Not Detected Not Detected
Bovine Parvovirus NA Not Detected Not Detected
Bovine Respiratory Syncytial Virus NA Not Detected Not Detected
Bovine Viral Diarrhea Virus NA Not Detected Not Detected
Rabies NA Not Detected Not Detected
Reovirus NA Not Detected Not Detected
Cytopathogenic Agents (IBR) NA Not Detected Not Detected
Hemadsorbing Agents (PI3) NA Not Detected Not Detected
Bluetongue NA Not Detected Not Detected

 

Additional information

Size

1 x 50 ml, 1 x 500 ml

18 reviews for Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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