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PurMa Charcoal-Stripped Fetal Bovine Serum (CS-FBS) 500 mL bottle, catalog number P3F015306-500
  • ✓Proudly made in the USA, manufactured in house
  • ✓Certificate of Analysis available for every lot
  • ✓Backed by decades of cell culture and protein biochemistry expertise
  • ✓Bulk pricing, purchase orders, and custom formulations welcome
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SKU: N/A Categories: Fetal Bovine Serum, Bovine Serums Tags: Charcoal-Stripped, Fetal Bovine Serum

Charcoal-Stripped Fetal Bovine Serum (CS-FBS)

Fetal bovine serum treated with activated charcoal to remove hormones and steroids.

  • Activated-carbon stripping removes steroids and hormones
  • For studies where background hormones or steroids must be minimized
SKU: N/A Categories: Fetal Bovine Serum, Bovine Serums Tags: Charcoal-Stripped, Fetal Bovine Serum

Description

Background

Charcoal stripped FBS (CS-FBS) is fetal bovine serum treated with activated carbon to remove hormones, steroids, and other lipophilic substances.

  • This process removes all the non-polar material such as lipophilic (lipid-related) materials (viruses, certain growth factors, hormones, and cytokines).
  • Acts as an effective blocker agent in immunoassay systems as well as immunoprecipitating analysis (by coating the Sepharose-activated beads in order to prevent nonspecific binding).
  • Moreover, it performs the function regardless of the molecular weight of the target.

PurMa Biologics applies Norit A charcoal coated with Dextran T-70 to manufacture a high-quality Charcoal-Stripped Fetal Bovine Serum.

Not sure which serum fits your cells? See our complete guide to cell culture serum.

PurMa™ Charcoal-Stripped FBS (CS-FBS)

  • PurMaTM Premium FBS is of USA origin.
  • Goes through heat inactivation and gamma irradiation.
  • Following the stripping, the FBS goes through heat inactivation process.
  • PurMa Biologics performs vigorous analysis following the stripping and will provide a certificate of analysis.
  • The procedure depletes any residue of  mycoplasma, steroids and hormones, and other pathogens.
  • Acceptable level of endotoxin (10 mg/Dl) and hemoglobin (20 mg/Dl).
  • Due to the loss of a vast variety of hormones and steroids (such as growth factors), we recommend using 15-20% CS-FBS in your media to ensure cultured cells’ wellbeing.

Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums

Further Instructions

On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.

References

  1. The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. Lippman et al. Cancer Res. 1976 Dec;36(12):4595-601. PMID:
  2. Leake et al. Steroid Hormones: A Practical Approach. Oxford, UK: IRL Press; 1987.
  3. Effects of estrogens and antiestrogens on estrogen receptor dynamics and the induction of progesterone receptor in MCF-7 human breast cancer cells. Eckert et al. Cancer Res. 1982 Jan;42(1):139-44.
Methodology Unit measured Accepted level Result
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) NA Not Detected Not Detected
pH N/A measured 6.58
Osmolality (USP 785)     mOsm/KgH2 mOsm/KgH20 280-340 288
Hemoglobin (Fleming & Woolf) mg/dL <25 12.9
Endotoxin (USP 85) EU/mL <20 <0.100
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) NA Not Detected Not Detected
IgG Elliza Measured 0.19 ug/ml
Albumin Eliza Measured ≤ 4.6 g/dL
Virus Testing (9 CFR 113.53c)
Bovine Adenovirus NA Not Detected Not Detected
Bovine Parvovirus NA Not Detected Not Detected
Bovine Respiratory Syncytial Virus NA Not Detected Not Detected
Bovine Viral Diarrhea Virus NA Not Detected Not Detected
Rabies NA Not Detected Not Detected
Reovirus NA Not Detected Not Detected
Cytopathogenic Agents (IBR) NA Not Detected Not Detected
Hemadsorbing Agents (PI3) NA Not Detected Not Detected
Bluetongue NA Not Detected Not Detected

 

Learn more: What Is Fetal Bovine Serum Used For?

Additional information

Size

1 x 50 ml, 1 x 500 ml

Documents

Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.

Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.

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Frequently asked questions

What sizes does CS-FBS come in?

Charcoal-Stripped Fetal Bovine Serum (CS-FBS) is supplied in 50 mL and 500 mL bottles.

What does charcoal stripping remove from FBS?

Dextran coated charcoal treatment strips small lipophilic molecules, principally steroid hormones, plus some growth factors and cytokines. What remains supports culture with a very low endogenous hormone background.

When should I use charcoal stripped FBS?

Use it for hormone response work where serum steroids would mask the effect you are measuring: oestrogen and androgen receptor studies, nuclear receptor assays, and any dosing experiment that needs a clean hormone baseline.

Is CS-FBS tested, and is a Certificate of Analysis available?

Yes. It is manufactured in house in the USA and each lot ships with a Certificate of Analysis available on request. Lot specific storage and expiry are printed on the bottle and listed on the COA.

Do you offer bulk pricing and purchase orders for CS-FBS?

Yes. Custom formulations, non standard fill volumes and bulk orders are all available, since everything is made in house. Purchase orders are accepted and bulk pricing is available. Request a quote from this page and we will come back with pricing and lead time.

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