Advanced Roswell-Park Memorial Institute 1640 (A-RPMI-1640)

Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-RPMI-1640 needs 2-5% FBS and provides more consistency.

Description

Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640)

Background

PurMaTM Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-RPMI-1640, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Contents

Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-RPMI-1640 incorporates the elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Additionally, Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) is a significant step toward a chemically defined form of this media. Chemically Defined Advanced RPMI-1640 (CD-A-RPMI-1640) is the outcome of decades of cutting-edge research. It will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here:  Advanced RPMI-1640 Formulation

References

  1. Culture of normal human leukocytes. Moore GE, et al. 1967 Feb 20;199(8):519-24. PMID: 4960081
  2. Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. Dunne HW, et al. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.
  3. Long-term culture of human leukaemic leukocytes. Pössnerová V, et al. 1970;17(5):513-23. PMID: 5274436
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A- RPMI-1640 Standard Formulation, A- RPMI-1640 w/o Sodium Bicarbonate, High HEPES (5.9 g/L), A- RPMI-1640 w/o L-Alanyl- L-Glutamine, A- RPMI-1640 w/o Phenol Red, A- RPMI-1640 w/o Sodium Bicarbonate, A- RPMI-1640 w/o Sodium Pyruvate, A- RPMI-1640 w/o Glucose, A- RPMI-1640 w/o Lipoic Acid, A- RPMI-1640 w/o L-Alanyl- L-Glutamine, w/o Phenol Red, A- RPMI-1640 w/o Phenol Red, w/o Sodium Bicarbonate, A- RPMI-1640 w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A- RPMI-1640 w/o L-Alanyl- L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A- RPMI-1640 w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A- RPMI-1640 w/o L-Alanyl- L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A- RPMI-1640 With Low Glucose (1.5 g/L), A- RPMI-1640 With High Sodium Bicarbonate (3.7 g/L), A- RPMI-1640 With 15 mM HEPES (3.6 g/L), A- RPMI-1640 With 25 mM HEPES (5.9 g/L), A- RPMI-1640 With 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (3.7 g/L), A- RPMI-1640 With 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (3.7 g/L), A- RPMI-1640 w/o Sodium Pyruvate, w/o Phenol Red, A- RPMI-1640 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), A- RPMI-1640 w/o Phenol Red, with 25 mM HEPES (5.9 g/L), A- RPMI-1640 w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A- RPMI-1640 w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), A- RPMI-1640 w/o Phenol Red, High Sodium Bicarbonate (3.7 g/L), A- RPMI-1640 w/o Sodium Pyruvate, High Sodium Bicarbonate (3.7 g/L), A- RPMI-1640 w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (3.7 g/L), A- RPMI-1640 w/o Sodium Pyruvate, w/o Phenol Red, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (3.7 g/L), A- RPMI-1640 w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A- RPMI-1640 w/o Phenol Red, w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), A- RPMI-1640 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (3.7 g/L), A- RPMI-1640 w/o Phenol Red, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (3.7 g/L), A- RPMI-1640 w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (3.7 g/L), A- RPMI-1640 w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (3.7 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Advanced RPMI-1640

SDS

SDS

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