Sale!
Basal Medium Eagle (BME) in Earl’s Buffer

Basal Medium Eagle (BME) in Earle’s Buffer

Basal Medium Eagle (BME) in Earl’s Buffer developed by Harry Eagle, and certainly is one of the most widely used of all synthetic cell culture media. BME  has demonstrated widely applicable  for supporting monolayer cell  growth in wide variety of normal as well as transformed cell lines.

SKU: N/A Categories: , Tags: , , , , , ,

Description

Basal Medium Eagle (BME) in Earle’s Buffer

Background

Basal Medium Eagle (BME) in Earle’s Buffer originally developed by Harry Eagle. this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. Moreover, PurMa Biologics manufacturers BME with Earle’s as well as in Hanks’ salts for use without CO2.

Ingredients

PurMa™ BME media in Earle’s buffer contains:

Earl’s buffer includes:

  1. Calcium chloride
  2. Magnesium sulfate
  3. Potassium chloride
  4. Sodium bicarbonate
  5. Sodium chloride
  6. and lastly, Sodium Phosphate, monobasic

PurMa Biologics so far, manufactures 109 types BME in Earle’s buffer.

Why PurMa Biologics? Why we stand behind our cell culture media.

Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. undoubtly, considering the highest quality, our prices certainly are extremely reasonable.

Application

BME provides an excellent nutritional capacity for suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts as well as  primary rat astrocytes.

Formulation

for complete formulation click here, alternatively you can find it in “Formulation Tab”:  BME with Earle’s Buffer Formulation

References:

  1. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
  2. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive N/A
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

BME Standard Formulation, BME w/o Glutamine, BME w/o Phenol Red, BME w/o Sodium Bicarbonate, BME w/o Glucose, BME w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, BME 15 mM HEPES (3.6 g/L), BME 25 mM HEPES (5.9 g/L), BME With Sodium Pyruvate (0.11 g/L), BME Low Sodium Bicarbonate (1.2 g/L), BME High Glucose (4.5 g/L), BME 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), BME 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.2g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

Quality Control

[vc-quality-control-tab]

Formulation

BME with Earl’s BufferFormulation

SDS

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.

Related products

  • McCoy’s 5A Modified Media MAX (M5AMM-MAX) Quick View
  • Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX) Quick View
  • Iscove’s Modified Dulbecco’s Medium (IMDM) Quick View