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Eagle's Minimal Essential Medium (EMEM)

Eagle’s Minimal Essential Medium (EMEM)

(18 customer reviews)

Eagle’s Minimal Essential Medium (EMEM) is suitable for adhesive as well as suspension cell lines.  PurMa Biologics manufactures 106 types of this medium

SKU: N/A Categories: Cell Culture Media, Eagle's Minimal Essential Medium (EMEM) Tags: Cell Culture, Phenol Red, Standard

Description

Background

Eagle’s Minimal Essential Medium (EMEM) developed by Harry Eagle and is widely used. It is composed of necessary salts for optimal cell growth and maintenance. Additionally, it contains high glucose, essential amino acids, and vitamins. Ever since, several versions of EMEM have been developed by modification of its composition. This media has is suitable for a wide range of mammalian cells, adhesive as well as suspension cells.

Ingridients

Moreover, PurMa TM EMEM Medium contains:

  • Low level of glucose (1.00 g/ liter)
  • Sodium bicarbonate (1.5 g/L)
  • Additionally, PurMa Biologics also offers EMEM Medium with high sodium bicarbonate (2.438 g/L) (Cat # P3S032110)
  • 2 mM L-glutamine (292 mg/L)
  • 1mM sodium pyruvate (0.11g/L)
  • Additionally, a higher concentration of amino acids, vitamins, inositol and choline are present in this medium.

PurMa Biologics manufactures 106 types of EMEM which accommodate any form of this medium. We recommend contacting our friendly customer service & technical support about your exact need for EMEM media.

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.

We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media.

We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.

Considering the highest quality, our prices are extremely reasonable.

Application of EMEM

Additionally, this media has elements that prevent clumping. This property certainly makes this media suitable for growing tumor cells as well as hybridoma cells lines. The dedicated scientists in PurMa Biologics have decades of experience with this media for culturing primary as well as commercial cell lines including human Osteosarcoma, Hek293 Cell, HeLa, Jurkat, MG63 cells, MCF-7, PC12, PBMC, and hybridoma cells.

Formulation

for complete formulation click here:  EMEM Formultation 

References

  1. Effects of assay medium composition on macrophage-mediated tumor cell binding and lysis. Hasday et al. J Immunol Methods. 1988 Nov 10;114(1-2):243-52. doi: 10.1016/0022-1759(88)90180-9. PMID: 3141516.
  2. Comparative activity in different media of ketoconazole, miconazole and amphotericin B against Candida lusitaniae and sucrose-negative Candida tropicalis. Mignini et al. Chemioterapia. 1988 Feb;7(1):38-41. PMID: 3378274.
  3. Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski et al. Folia Histochem Cytobiol. 1988;26(3):123-31. PMID: 2461881.
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

EMEM Standard Formulation, EMEM w/o Glutamine, EMEM w/o Phenol Red, EMEM w/o Sodium Bicarbonate, EMEM w/o Sodium Pyruvate, EMEM w/o Glucose, EMEM w/o Glutamine, w/o Phenol Red, EMEM w/o Phenol Red, w/o Sodium Bicarbonate, EMEM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, EMEM w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, EMEM High Glucose (4.5 g/L), EMEM High Sodium Bicarbonate (2.438 g/L), EMEM High Glucose (4.5 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM 15 mM HEPES (3.6 g/L), EMEM 25 mM HEPES (5.9 g/L), EMEM w/o Sodium Pyruvate, w/o Phenol Red, EMEM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), EMEM w/o Phenol Red, with 25 mM HEPES (5.9 g/L), EMEM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

18 reviews for Eagle’s Minimal Essential Medium (EMEM)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

Only logged in customers who have purchased this product may leave a review.

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