Description

HEPES Stock Buffer Solution

Background

HEPES Stock Buffer Solution is one of the commonly used buffers in cell cultures as well as biochemical analysis. HEPES Maintains psychological pH despite changes in carbon dioxide concentration, therefore HEPES it acts better than bicarbonate in cell culture applications.  However, HEPES shows an unwanted photochemical process in presence of riboflavin when exposed to ambient light to produce hydrogen peroxide. We therefore recommend keeping the mentioned solutions in dark.

Grades of HEPES Stock Buffer Solution

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

we finally sterilize the buffers by autoclaving the solution and packing  in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2

The Endotoxin Removing Procedure from Buffer

PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer  through the PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B   to an endotoxin-specific absorbent. we finally  eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

Features

• Cell Culture Suited
• Provides a buffer solution to maintain the physiological pH despite changes in carbon dioxide concentration.
• PurMa Biologics manufactures this buffer  using Mg²⁺ and Ca ²⁺free water
• Free of RNAse /DNase
• Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
• We rigorously test for contamination of endotoxin and mycoplasma.
• PurMa Biologics manufactures this buffer in several PH formats and NaOH adjusts the pH.
• We Sterilize this buffer using  0.2µm filters.
• As it is packed under N_2, the pH won’t change over time before opening the bottle.

References

1. Toxicity of light-exposed Hepes media. Lepe-Zuniga JL, Zigler JS Jr, Gery I. J Immunol Methods. 1987 Oct 23;103(1):145. doi: 10.1016/0022-1759(87)90253-5. PMID: 3655381.
2. Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium. Zigler, JS Jr , Lepe-Zuniga JL, Vistica B, Gery I. In Vitro Cell Dev Biol. 1985 May;21(5):282-7. doi: 10.1007/BF02620943. PMID: 4019356.