Iscove’s Modified Dulbecco’s Medium (IMDM)

Although, Iscove’s Modified Dulbecco’s Medium (IMDM) is specialized for rapidly proliferating, cell lines, it certainly quite suitable for slow growing cells as well. Moreover, this medium is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate , therefore makes it an excellent choice for adhesive cells.

Description

Iscove’s Modified Dulbecco’s Medium (IMDM)

Background

Iscove’s Modified Dulbecco’s Medium (IMDM) is specialized for rapidly proliferating, high-density cell lines. Therefore, it is excellent for Jurkat, Hek, COS-7, and macrophage cells. IMDM is a modified and more advanced version of DMEM. Additionally, it includes selenium as well as additional amino acids and vitamins. it is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate, therefore it makes it excellent  for adhesive cells.

Ingredients

It contains:

  • High Buffering Capacity with High NaHCO3 as well as  High HEPES
  • Sodium Bicarbonate (3.02 g/L)
  • 5.9 g/L HEPES
  • High glucose 4.5g/L
  • Non-Essential Amino Acids
  • 110 mg/L Sodium pyruvate (1.0 mM)
  • 15 mg/L Phenol Red
  • 0.584 g/L L-glutamine

Formulation

Complete formulation is available on our website as well as here : IMDM Formulation 

References

  1. Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
  2. Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

IMDM Standard Formulation, IMDM w/o Glutamine, IMDM w/o Phenol Red, IMDM w/o Sodium Bicarbonate, IMDM w/o Sodium Pyruvate, IMDM w/o Glucose, IMDM w/o HEPES, IMDM w/o Glutamine, w/o Phenol Red, IMDM w/o Phenol Red, w/o Sodium Bicarbonate, IMDM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, IMDM w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, IMDM w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, IMDM w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, IMDM Low Glucose (1.5 g/L), IMDM Low Sodium Bicarbonate (1.5 g/L), IMDM 15 mM HEPES (3.6 g/L), IMDM 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), IMDM w/o Sodium Pyruvate, w/o Phenol Red, IMDM w/o Sodium Pyruvate, w/o HEPES, IMDM w/o phenol Red, w/o Sodium Pyruvate, w/o HEPES, IMDM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), IMDM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), IMDM w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), IMDM w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), IMDM w/o Phenol Red, w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), IMDM w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), IMDM w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), IMDM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), IMDM w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

IMDM-Standard Formulation

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.