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Whole Tissue Cryoprotective Media (WTCM); DMSO Free

Whole Tissue Cryoprotective Media (WTCM); DMSO Free

(18 customer reviews)

Cryopreservation of tissues especially when are big could be challenging. Moreover, the biggest obstacle is forming ice crystals. As a result, it causes volume expansion with the destruction of cell membranes. Whole Tissue Cryoprotective Media (WTCM); DMSO Free properly penetrates in the tissue and rapidly lowers the temperature inside the tissue. Under the DMSO Free condition, tissues must be frozen at the rate of 1°C/ min.

SKU: N/A Categories: Cryoprotective Media, Cryoprotective media DMSO-Free Tags: Cryoprotective Media, DMSO Free, Whole Tissue

Description

Background

Cryopreservation of tissues especially when are big could be challenging. Moreover, the biggest obstacle is forming ice crystals. Undeniably, it causes volume expansion with the destruction of cell membranes and holes in loose connective tissue. Whole Tissue Cryoprotective Media (WTCM); DMSO Free properly penetrates in the tissue and rapidly lowers the temperature inside the tissue. Under the DMSO Free condition, tissues must be frozen at the rate of 1°C/ min.

The crucial Role of the Rapid freezing in whole Tissue Cryoprotection

Tissues should be frozen as rapidly as possible to promote the formation of amorphous ice (rather than crystal ice). With low specific heat constant, liquid nitrogen when contacts warm tissues, it boils and forms a vapor barrier which acts as an insulator. Consequently, the inner core of the tissue sample freezes slowly and unevenly. The PurMa™ WTCM has high thermal conductivity and therefore, has overcome the mentioned problem.

Our Cryoprotective strategy

PurMa Biologics has developed a DMSO-free whole-tissue cryoprotective media which is the outcome of many years of cutting-edge science. The cryoprotectant in our media is a complex of heavy chain sugars. This combination adequately freezes down the tissue with minimal ice formation and extracellular hydration.

Please read our protocol before using DMSO Free PurMa™ WTCM Cryoprotective Media as following the protocol is crucial for proper cryopreservation.

PurMa™ Whole Tissue Cryoprotective Media (WTCM)

  • Ready-to-Use.
  • It is DMSO-Free.
  • PurMaTM WTCM provides much higher cell viability after short/long-term cryoprotection.
  • PurMaTM WTCM Prevents the formation of crystals in extracellular space, consequently, preventing cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.
  • Preserves morphological appearance as a standard factor of cell recovery.
  • Tested for pH, osmolality, sterility, and endotoxin.
  • Each Lot of PurMaTM WTCM besides the pathogens and sterility evaluations goes through a survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), and the rate of recovery by Alamar Blue test.
  • PurMaTM WTCM has been tested successfully for several commonly used tissue including rodent heart, brain, and kidney

Guideline

for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines

References

  1. Cryopreservation of whole ovine ovaries with pedicles as a model for human: parameters of perfusion with simultaneous saturations by cryoprotectants. Isachenko et al. Clin Lab. 2015;61(3-4):415-20. doi: 10.7754/clin.lab.2014.140919.
  2. Analysis of variations in cryomedium and methods of freezing on human bone marrow stem cells. O’Kane-Murphy et al.Prog Clin Biol Res. 1992;377:361-6.
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Size

1 x 10 ml, 1 x 100 ml

18 reviews for Whole Tissue Cryoprotective Media (WTCM); DMSO Free

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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