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R Cells Cryoprotective Media (RCCM) With DMSO

R Cells Cryoprotective Media (RCCM) With DMSO

(18 customer reviews)

PurMa™ R -Cell Cryoprotective Media (RCCM-D) performs highly for cryopreservation of reproductive cells such as Oocytes & Spermatozoa. It contains proprietary formulation including DMSO.

SKU: N/A Categories: Cryoprotective Media, Cryoprotective media With DMSO Tags: Cryoprotective Media, Oocytes, Spermatozoa

Description

Background

PurMa™ R -Cell Cryoprotective Media (RCCM-D) performs highly for cryopreservation of reproductive cells such as Oocytes & Spermatozoa. It contains proprietary formulation including DMSO. Exposing cells to temperatures below 0°C without the aid of cryoprotectants has two key consequences:

  1. Osmotic gradient forms across the cell membrane, which causes “cell dehydration”, and
  2. Ice formation and abnormal concentration of solutes in the intracellular matrix, osmatic shock, and toxicity.

PurMa Biologics, with decades of experience in cutting-edge science, has manufactured PurMa™ R Cells Cryoprotective Media (RCCM) With DMSO  which gives a high survival and recovery rate for reproductive cells.

There are several criteria that make DMSO a powerful cryoprotective agent

  1. PurMa™ R -Cell Cryoprotective Media (RCCM-D) provides strong molecular stability.
  2. Besides, facilitates a high non-conductor constant.
  3. It contains the basic properties, salts solubility particularly for anions. Additionally contains a propensity to act as the acceptor atom in hydrogen bonding.
  4. Especially, DMSO makes a  hydrogen bond with water which is 30% stronger than between two water molecules.
  5. The proprietary formulation of PurMa™ RCCM-D allows the cryoprotective agent getting inside the cells instantly and lower cell death because of penetration/freezing.

PurMa™ R -Cell Cryoprotective Media (RCCM-D) penetrates inside the cells instantly and lower cell death.

Special Features for R Cells Cryoprotective Media (RCCM) With DMSO

  • Ready-to-Use medium.
  • Each Lot of our PurMaTM RCCM-D with DMSO goes through pathogens and sterility evaluations.
  • We evaluate each Lot for survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), as well as for the rate of recovery by Alamar Blue test.
  • R Cells Cryoprotective Media (RCCM) With DMSO results in the lowest cell loss during freezing and thawing as well as the highest cell recovery. Moreover, it provides over 66% survival and recovery rate after long term cryopreservation.
  • Sterile (Passed through 0.2µm filters).
  • Moreover, PurMaTM RCCM-D provides an exceptional cryopreservation for primary and commercial cell lines.
  • Suitable for clinical applications.

Guideline R Cells Cryoprotective Media (RCCM) With DMSO

For PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines

References

  1. Fertilization of human oocytes following cryopreservation; normal karyotypes and absence of stray chromosomes. Gook et al. Hum Reprod. 1994 Apr;9(4):684-91. doi:10.1093/ oxfordjournals .humrep .a138572.
  2. Cryopreservation of single human spermatozoa. Cohen et al. Hum Reprod. 1997 May;12(5):994-1001. doi: 10.1093/humrep/12.5.994.
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Size

1 x 10 ml, 1 x 100 ml

18 reviews for R Cells Cryoprotective Media (RCCM) With DMSO

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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