Advanced Kaighn’s Modification of Ham’s F-12 (A-Ham’s F-12K)
Advanced Kaighn’s Modification of Ham’s F-12 Medium (A-Ham’s F-12K) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-Ham’s F-12K needs 2-5% FBS and provides more consistency.
Description
Advanced Kaighn’s Modification of Ham’s F-12 (A-Ham’s F-12K)
Background
PurMaTM Advanced Kaighn’s Modification of Ham’s F-12 Medium (A-Ham’s F-12K) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-Ham’s F-12K, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced Kaighn’s Modification of Ham’s F-12 Medium (A-Ham’s F-12K) Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-Ham’s F-12K incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Kaighn’s Modification of Ham’s F-12 Medium (A-Ham’s F-12K) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced Ham’s F-12K (CD-A-Ham’s F-12K) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced Ham’s-F12 Formulation
References
- Growth and continuous passage of COMMA-D mouse mammary epithelial cells in hormonally defined serum-free medium. Riss TL, Sirbasku DA. Cancer Res. 1987 Jul 15;47(14):3776-82. PMID: 3297308.
- Hormones and factors that stimulate growth of a rat islet tumor cell line in serum-free medium. Fong HK, et al. Diabetes. 1981 Dec;30(12):1022-8. doi: 10.2337/diab.30.12.1022. PMID: 6273246
- Primary in vitro antibody responses by purified murine B lymphocytes in serum-free defined medium. Mosier DE. J Immunol. 1981 Oct;127(4):1490-3. PMID: 6792277
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-HAM’S F-12K Standard Formulation, A-HAM’S F-12K w/o L-Alanyl-L-Glutamine, A-HAM’S F-12K w/o Phenol Red, A-HAM’S F-12K w/o Sodium Bicarbonate, A-HAM’S F-12K w/o Sodium Pyruvate, A-HAM’S F-12K w/o Glucose, A-HAM’S F-12K w/o Lipoic Acid, A-HAM’S F-12K w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-HAM’S F-12K w/o Phenol Red, w/o Sodium Bicarbonate, A-HAM’S F-12K w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-HAM’S F-12K w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-HAM’S F-12K w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-HAM’S F-12K w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-HAM’S F-12K High Glucose (4.5 g/L), A-HAM’S F-12K Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K High Sodium Bicarbonate (3.7 g/L), A-HAM’S F-12K 15 mM HEPES (3.6 g/L), A-HAM’S F-12K 25 mM HEPES (5.9 g/L), A-HAM’S F-12K 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, A-HAM’S F-12K w/o Phenol Red, with 15 mM HEPES (3.6 g/L), A-HAM’S F-12K w/o Phenol Red, with 25 mM HEPES (5.9 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), A-HAM’S F-12K w/o Phenol Red, Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, with 15 mM HEPES (3.6 g/L) Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, w/o Phenol Red, with 25 mM HEPES (5.9 g/L) Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Phenol Red, w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), A-HAM’S F-12K w/o Phenol Red, w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), A-HAM’S F-12K w/o Phenol Red, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Phenol Red, with 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-HAM’S F-12K w/o Sodium Pyruvate, with 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 1 X 10L ( Powder), 1 X 50 L (Powder), 10 X 1EZ Individually Packed (Powder), 50 X 1EZ Individually Packed (Powder), 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml |
Quality Control
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