PurMaTM Advanced Ham-F12contains components that allow for a 60–90% reduction in FBS supplementation. Using synthetic components such as insulin recombinant full chain, recombinant albumin, and transferrin increase consistency in results with less variation amongst lot-to-lot serum changes.  In Advanced HAM-F12, L‑alanine‑L‑glutamine is used as the source of glutamine.  Additionally, reducing FBS requirements lowers the cost of cell culture experiments. Advanced Ham-F12 contains:

  • All the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • It includes the elements which significantly reduce the need for a serum for cell culture including:

Advanced HAM-F12 is a substantial upgrade to the standard and MAX for of HAM-F12. That is because media this media contains ingredients to allow for serum reduction, specifically: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, lipid rich PurMaTM Albumin, trace elements such as sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

  1. PurMaTM Albumin:  Chromatographically purified with IgG content <=0.1.0%.
  1. PurMaTM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  2. PurMaTM Insulin Recombinant
  3. Trace of other elements which mimic the elements in fetal bovine serum.

In another word, advanced form of HAM-F12 is a significant step toward chemically defined form of this media where all elements are synthetic and the need for FBS is eliminated.

Chemically Defined HAM-F12 (CD- HAM-F12) is the next generation of PurMa Biologics’ dedicated team of top scientists which is the outcome of decades of cutting-edge research. CD- HAM-F12 soon will be offered globally to laboratories who are looking for consistency and repeatability among their data.

Formulation: Complete formulation is available here: Advanced Ham’s F-12K

Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature