Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer

Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer contains L-Alanyl-L-Glutamine as well as serum-reducing ingredients. Additionally, Advanced BME needs 2-5% FBS and it furthermore provides more consistency.

Description

Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer

Background

PurMaTM Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in Advanced BME. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.

Contents

Advanced BME in HBSS Contains:

  • It includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-BME incorporates elements to significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems, which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced BME (CD-A-BME) is the outcome of decades of cutting-edge research.  Furthermore, it will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

The complete formulation can be found here: Advanced BME in Hanks’ Buffer (HBSS) Formulation

References

  1. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
  2. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A-BME (HBSS) Standard Formulation, A-BME (HBSS) w/o L-Alanyl-L-Glutamine, A-BME (HBSS) w/o Phenol Red, A-BME (HBSS) w/o Sodium Bicarbonate, A-BME (HBSS) w/o Glucose, A-BME (HBSS) w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-BME (HBSS) w/o L-Alanyl-L-Glutamine, w/o Sodium Bicarbonate, A-BME (HBSS) w/o Phenol Red, w/o Sodium Bicarbonate, A-BME (HBSS) w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-BME (HBSS) 15 mM HEPES (3.6 g/L), A-BME (HBSS) 25 mM HEPES (5.9 g/L), A-BME (HBSS) High Sodium Bicarbonate (0.85 g/L), A-BME (HBSS) High Glucose (4.5 g/L), A-BME (HBSS) 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (0.85 g/L), A-BME (HBSS) 25 mM HEPES (3.6 g/L), High Sodium Bicarbonate (0.85 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

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Formulation

Advanced BME in Hanks’ Buffer (HBSS)

 

SDS

SDS

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