RPMI-1640 was originally developed in 1967 by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. It has traditionally been used for the growth of human lymphocytes. This medium contains a great deal of phosphate which is an essential element for tumor cells due to using glycolysis as a main source of energy in cell aggregates which is one of the most useful strategies for tumor cells survival.

There are three common formulations for standard RPMI-1640 which currently used in laboratories across the globe. PurMa Biologics manufactures all three types of this media routinely. The difference among these formulations is exclusively the buffering capacity. Our standard formulation is the following first option, and the other two can be ordered by choosing the corresponding variations from the “Conditions” drop down. Please find the complete formulation in “Formulation” tab we recommend if you have been using media from other vendors, contact our friendly customer service & technical support to exactly advise you what media you should exactly should be using to make %100 match with the media you have been using:

  • Containing 2.00 g/L NaHCO3 and without g/L HEPES (Our Standard formulation)
  • PurMa Biologics offers more buffering capacity as the following:
  1. With15 mM HEPES (3.6 g/L) Cat #P3S026109
  2. With25 mM HEPES (5.9 g/L). Cat #P3S027109
  3. With 3.7 g/L NaHCO3. Cat #P3S032109
  4. With 3.7 g/L NaHCO3, & With15 mM HEPES (3.6 g/L). Cat #P3S072109
  5. With 3.7 g/L NaHCO3, & With 25 mM HEPES (5.9 g/L). Cat #P3S073109

Why PurMa Biologics? Why we stand behind our cell culture media:

  1. Our products are the outcome of 30 years of experience in cutting edge cell and tissue culture science.
  2. We routinely manufacture over 1500 media which is the most comprehensive collection of cell and tissue culture media.
  3. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab compared to other laboratories working on the same issues.
  4. Considering the highest quality, our prices are extremely reasonable.

ApRegular RPMI-1640 Formulation109

RPMI1640 has been formulated for use in a 5% carbon dioxide atmosphere and is not recommended for higher and lower percentages of CO2. It utilizes a bicarbonate buffering system. Scientists in PurMa Biologics have successfully evaluated RPMI-1640 medium for the culture of human normal and neoplastic leukocytes, HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, carcinomas, bone marrow, and hybridoma cells. The major difference between RPMI1640 and other media besides higher PH (8) is, it contains biotin, vitamin B12, and PABA. In addition to alterations in the amounts of amino acids and vitamins, inositol, and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

Formulation: Complete formulation is here: RPMI-1640-Formulation

  1. Culture of normal human leukocytes. Moore GE, Gerner RE, Franklin HA. 1967 Feb 20;199(8):519-24. PMID: 4960081
  2. Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. Dunne HW, Kmetz M, Schultz RD, Zimmerer RP, Yilmazer SK. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.
  3. Long-term culture of human leukaemic leukocytes. Pössnerová V, Smetana K, Krecek M, Hermanský F, Fortýnová J. 1970;17(5):513-23. PMID: 5274436
  4. In vitro cultivation of adult Litomosoides carinii: evaluation of basic culture media, gas phases and supplements. Mössinger J. 1991 Aug;103 Pt 1:85-95. doi: 10.1017/s0031182000059321. PMID: 1945528
  5. Genotoxic effect of formocresol pulp therapy of deciduous teeth. Lucas Leite ACG, Rosenblatt A, da Silva Calixto M, da Silva CM, Santos N. Mutat Res. 2012 Aug 30;747(1):93-97. doi: 10.1016/j.mrgentox.2012.04.006. Epub 2012 May 11. PMID: 22579796
  6. Formocresol mutagenicity following primary tooth pulp therapy: an in vivo study. Zarzar PA, Rosenblatt A, Takahashi CS, Takeuchi PL, Costa Júnior LA. J Dent. 2003 Sep;31(7):479-85. doi: 10.1016/s0300-5712(03)00087-3. PMID: 12927459
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature