Advanced MEM Alpha-Modification (A-MEM-α) in Earle’s Buffer

Advanced MEM Alpha-Modification (A-MEM-α) in Earle’s Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction.  Additionally, A-MEM-α needs 2-5% FBS and provides more consistency.

Description

Advanced MEM Alpha-Modification (A-MEM-α) in Earle’s Buffer

Background

PurMaTM Advanced MEM Alpha-Modification (A-MEM-α) in Earle’s Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. Consequently, this results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in Advanced MEM-α. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.

Contents

Advanced MEM-α in EBSS Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-MEM-α incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced MEM Alpha-Modification (A-MEM-α) in Earle’s Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM-α (CD-A-MEM-α) is the outcome of decades of innovative cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced MEM-alpha in Earle’s Buffer 

References

  1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
  2. Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, Wun CC, Grunert GM. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
  3. Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham’s F-10. Roudebush WE, Often NL, Butler WJ. J Assist Reprod Genet. 1994 Apr;11(4):203-7. doi: 10.1007/BF02211809. PMID: 7711383.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A-MEM-⍺ Standard Formulation, A-MEM-⍺ w/o L-Alanyl-L-Glutamine, A-MEM-⍺ w/o Phenol Red, A-MEM-⍺ w/o Sodium Bicarbonate, A-MEM-⍺ w/o Glucose, A-MEM-⍺ w/o Lipoic Acid, A-MEM-⍺ w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-MEM-⍺ w/o L-Alanyl-L-Glutamine, w/o Sodium Bicarbonate, A-MEM-⍺ w/o Phenol Red, w/o Sodium Bicarbonate, A-MEM-⍺ w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-MEM-⍺ 15 mM HEPES (3.6 g/L), A-MEM-⍺ 25 mM HEPES (5.9 g/L), A-MEM-⍺ w/o Sodium Pyruvate, A-MEM-⍺ High Glucose (4.5 g/L), A-MEM-⍺ High Sodium Bicarbonate (3.7 g/L), A-MEM-⍺ Low Sodium Bicarbonate (0.85 g/L), A-MEM-⍺ 15 mM HEPES (3.6 g/L), w/o Glucose, A-MEM-⍺ 25 mM HEPES (5.9 g/L), w/o Glucose, A-MEM-⍺ 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), A-MEM-⍺ 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), A-MEM-⍺ 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.85 g/L), A-MEM-⍺ 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.85 g/L), A-MEM-⍺ 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (3.7 g/L), A-MEM-⍺ 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (3.7 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

Advanced MEM- Alpha in Earl’s Buffer Formulation

 

SDS

SDS

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