MEM Alpha-Modification MAX (MEM-α-MAX) in Hanks’ Buffer
$50.99 – $1109.66
MEM Alpha-Modification MAX (MEM-α-MAX) in Hanks’ Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. MEM-α-MAX in HBBS significantly improves cell viability and growth. We manufacture several types of MEM-α-MAX based on your requirements.
Description
MEM Alpha-Modification MAX (MEM-α-MAX) in Hanks’ Buffer
Background
MEM Alpha-Modification MAX (MEM-α-MAX) in Hanks’ Buffer is a commonly used media for Mammalian cell culture.
Advantages
MEM-α performs better than MEM for a number of cell lines, please see the description of regular MEM-α : MEM-α
The advantages of MEM-α-MAX in HBBS over MEM-α are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.
In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.
Contents
MEM Alpha-Modification MAX (MEM-α-MAX) in Hanks’ Buffer contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of MEM-α-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
MEM-α-MAX in HBBS contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).
Formulation
For complete formulation Click here: MEM-alpha-MAX Formulation in Hanks’ Buffer (HBSS)
References
- The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
- Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, et al. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
- Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham’s F-10. Roudebush WE, et al. J Assist Reprod Genet. 1994 Apr;11(4):203-7. doi: 10.1007/BF02211809. PMID: 7711383.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | MEM-α-MAX (HBSS) Standard Formulation, MEM-α-MAX (HBSS) w/o Phenol Red, MEM-α-MAX (HBSS) w/o Sodium Bicarbonate, MEM-α-MAX (HBSS) w/o Glucose, MEM-α-MAX (HBSS) w/o Lipoic Acid, MEM-α-MAX (HBSS) w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α-MAX (HBSS) 15 mM HEPES (3.6 g/L), MEM-α-MAX (HBSS) 25 mM HEPES (5.9 g/L), MEM-α-MAX (HBSS) w/o Sodium Pyruvate, MEM-α-MAX (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM-α-MAX (HBSS) High Glucose (4.5 g/L), MEM-α-MAX (HBSS) 15 mM HEPES (3.6 g/L), w/o Glucose, MEM-α-MAX (HBSS) 25 mM HEPES (5.9 g/L), w/o Glucose, MEM-α-MAX (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-α-MAX (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), MEM-α-MAX (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L), MEM-α-MAX (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), High Sodium Bicarbonate (0.85 g/L), MEM-α-MAX (HBSS) 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (0.85 g/L), MEM-α-MAX (HBSS) 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (0.85 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed |
Quality Control
[vc-quality-control-tab]Formulation
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