Charcoal-Stripped Fetal Bovine Serum (CS-FBS)

Description

Background

Charcoal stripped FBS (CS-FBS) is fetal bovine serum treated with activated carbon to remove hormones, steroids, and other lipophilic substances.

• This process removes all the non-polar material such as lipophilic (lipid-related) materials (viruses, certain growth factors, hormones, and cytokines).
• Acts as an effective blocker agent in immunoassay systems as well as immunoprecipitating analysis (by coating the Sepharose-activated beads in order to prevent nonspecific binding).
• Moreover, it performs the function regardless of the molecular weight of the target.

PurMa Biologics applies Norit A charcoal coated with Dextran T-70 to manufacture a high-quality Charcoal-Stripped Fetal Bovine Serum.

Not sure which serum fits your cells? See our complete guide to cell culture serum.

PurMa™ Charcoal-Stripped FBS (CS-FBS)

• PurMa^TM Premium FBS is of USA origin.
• Goes through heat inactivation and gamma irradiation.
• Following the stripping, the FBS goes through heat inactivation process.
• PurMa Biologics performs vigorous analysis following the stripping and will provide a certificate of analysis.
• The procedure depletes any residue of  mycoplasma, steroids and hormones, and other pathogens.
• Acceptable level of endotoxin (10 mg/Dl) and hemoglobin (20 mg/Dl).
• Due to the loss of a vast variety of hormones and steroids (such as growth factors), we recommend using 15-20% CS-FBS in your media to ensure cultured cells’ wellbeing.

Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums

Further Instructions

On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.

References

1. The effects of estrogens and antiestrogens on hormone-responsive human breast cancer in long-term tissue culture. Lippman et al. Cancer Res. 1976 Dec;36(12):4595-601. PMID:
2. Leake et al. Steroid Hormones: A Practical Approach. Oxford, UK: IRL Press; 1987.
3. Effects of estrogens and antiestrogens on estrogen receptor dynamics and the induction of progesterone receptor in MCF-7 human breast cancer cells. Eckert et al. Cancer Res. 1982 Jan;42(1):139-44.