Description

Charcoal-Stripped Fetal Horse Serum (CS-FHS)

Background

PurMa Biologics manufactures PurMa™ Charcoal-Stripped Fetal Horse Serum (CS-FHS) by treating the Fetal Horse Serum with activated carbon is an effective process for removing hormones and steroids.

• This process removes all the non-polar material such as lipophilic (lipid-related) materials (virus, certain growth factors, hormones, and cytokines).
• It is an effective blocker agent in immunoassay systems as well as immunoprecipitating analysis (by coating the Sepharose-activated beads to prevent nonspecific binding).
• Regardless of the molecular weight of the target.

PurMa Biologics applies Norit A charcoal coated with Dextran T-70 manufactures a high-quality Charcoal-Stripped Fetal Horse Serum.

PurMa™ Charcoal-Stripped FHS (DFHS)

• PurMa^TM Premium FHS is USA origin.
• The parent FHS has been gamma irradiated.
• Following the stripping, the FHS goes through heat inactivation process.
• Following the stripping, the FHS goes through vigorous analysis and will get a certificate of analysis.
• It is free of mycoplasma, steroids and hormones, and other pathogens.
• Acceptable level of endotoxin (10 mg/Dl) and hemoglobin (20 mg/Dl).
• Due to the loss of a vast variety of hormones and steroids (such as growth factors), we recommend using 15-20% CS-FBS in your media to make sure cultured cells are well beings.

Further Instructions

On receiving the bottle or on first usage, defrost and aliquot the bottle into smaller quantities as freezing and thawing is not recommended for this product.

Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums

References

1. Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
2. Estrogen mitogenic action. I. Demonstration of estrogen-dependent MTW9/PL2 carcinogen-induced rat mammary tumor cell growth in serum-supplemented culture and technical implications. Moreno-Cuevas et al. In Vitro Cell Dev Biol Anim. 2000 Jul-Aug;36(7):410-27.