Description

Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)

Background

PurMa Biologics manufactures seven types of bovine serum, three of which however, considered as premium fetal bovine serums, each specialized for certain requirements in tissue culture. Moreover,  We highly evaluate Endotoxin-Depleted Fetal Bovine Serum (ED-FBS) for endotoxin and after triple filtering with 0.1µm,  the residual of endo toxin were depleted using PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251) ( see below).

PurMa^TM Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)

• PurMa^TM Endotoxin Depleted Fetal Bovine Serum (EDP-FBS) is of USA origin.
• Additionally, has gone through  an endotoxin depletion procedure.
• Has gone through  gamma irradiation as well as heat inactivation process.
• Lastly, we vigorously analyze various aspect and each LOT possess a  certificate of analysis.

Endotoxin Removal Procedure

1. PurMa Biologics processes PurMa^TM ED-FBS from PurMa^TM Premium Fetal Bovine Serum (P-FBS).  It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.
2. The process of removing endotoxin from FBS  routinely starts with passing the premium FBS through the PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251) with the rate of 0.1 ml/ minute.
3. The unbound fractions pass through 0.1µm filters  for three times. The mentioned procedure eliminates the residual of endotoxin as well.
4. We conjugate  Sepharose 6B in our proprietary column covalently to powerful endotoxin-specific absorbent.

Analysis

PurMa biologics routinely performs pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums

References

1. Biochemical and morphological studies of steady state and lipopolysaccaride treated bovine articular cartilage explant cultures. Tian et al. Connect Tissue Res. 1989;19(2-4):195-218. doi: 10.3109/03008208909043897. PMID:
2. The preparation of antigenic hapten-carrier conjugates: a survey. Erlanger BF. Methods Enzymol. 1980;70(A):85-104. doi: 10.1016/s0076-6879(80)70043-5.