Endotoxin-Depleted Fetal Horse Serum (ED-FHS)
PurMa Biologics manufactures seven types of horse serum, three of which, including PurMaTM Endotoxin-Depleted Fetal Horse Serum (EDP-FHS). Moreover, they all have the highest quality horse serums, each performs certain requirements in tissue culture.
Description
PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS)
Background
PurMa Biologics manufactures seven types of horse serum, three of which, including PurMaTM Endotoxin-Depleted Fetal Horse Serum (ED-FHS) are considered as premium fetal Horse serums, each specialized for certain requirements in tissue culture:
- PurMa™ Premium Fetal Horse Serum (P-FHS).
- PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS).
- PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS).
Additionally, PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS) is processed from PurMa™ Premium Fetal Horse Serum (P-FHS). It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.
Endotoxin Removal
Purma Biologics removes the endotoxin from FHS by passing the premium FHS through the PurMa™ Endotoxin Elimination Column (Cat# P6H1216251). The Sepharose 6B in our proprietary column is covalently conjugated to an endotoxin-specific absorbent. The mentioned procedure follows by eliminating the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.
PurMa™ Premium FBS
- Firstly, PurMa™Endotoxin Depleted Fetal Horse Serum (EDP-FHS) is of USA origin.
- Also, it is free of endotoxin.
- Moreover, it Has been gamma irradiated.
- Has been heat inactivated.
- and lastly, has been vigorously analyzed with the certificate of analysis
Routine pathological analysis on PurMa bovine and horse serums:
Reference
- Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
- Higher percentage of horse serum in culture media blocks attachment of PC12 cells. Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Quality Control
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