Glasgow’s Modified Eagle’s Medium
Glasgow’s Modified Eagle’s Medium was originally developed to study the polyoma virus. However, due to particular criteria has been mentioned in the long description, this media is used without serum.
Description
Glasgow’s Modified Eagle’s Medium
Background
Ian McPherson and Michael Stoker developed Glasgow’s Modified Eagle’s Medium . they used polyoma virus to transform four fibroblast clones from a culture of baby hamster kidney cells. as a result to Particular criteria mentioned below Glasgow Minimum Essential Medium (GMEM) is used without serum. Needs to be supplemented with 10% Tryptose phosphate broth.
Moreover, it contains twice amount of amino acids and vitamins compared to the original Basal Medium Eagle.
PurMa™ Glasgow’s Modified Eagle’s Medium also Contains
- 75 g/L Sodium Bicarbonate
- High Glucose (4.5g/L)
PurMa Biologics manufactures 77 types of GMEM
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.
Application of this media
It is suitable for growth of primate embryonic stem cells, MDCK, NS0, BHK-21 as well as CHO cells containing the GS expression system.
Alternative to 10% Tryptose phosphate broth, we strongly recommend PurMa TM 50X GS Supplement. This is certainly a enriched mixture of amino acids and nucleosides, and Fetal Bovine Serum (FBS) (Cat P3S528332). furthermore, this media has been formulated for use in a 5% carbon dioxide atmosphere. As a result, It utilizes a bicarbonate buffering system.
Formulation
The Complete formulation is available in ” formulation tab” as well as by clicking here: Glasgow’s (GMEM) Formulation
References
- The stimulation of Na+, K+-ATPase activity in the medulla of the rat kidney by [arginine] vasopressin and its analogues. Pippard et al. Clin Sci (Lond). 1984 May;66(5):561-7. doi: 10.1042/cs0660561. PMID:
- Uptake of glutamate, not glutamine synthetase, regulates adaptation of mammalian cells to glutamine-free medium. McDermott RH, Butler M. J Cell Sci. 1993 Jan;104 ( Pt 1):51-8. doi: 10.1242/jcs.104.1.51.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | GMEM Standard Formulation, GMEM w/o Phenol Red, GMEM w/o Sodium Bicarbonate, GMEM w/o Sodium Pyruvate, GMEM w/o Glucose, GMEM Low Glucose (1.5 g/L), GMEM Low Sodium Bicarbonate (0.35 g/L), GMEM Low Sodium Bicarbonate (1.1 g/L), GMEM 15 mM HEPES (3.6 g/L), GMEM 25 mM HEPES (5.9 g/L), GMEM 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), GMEM 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.1 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]SDS
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