McCoy’s 5A Modified Media (M5AMM)
McCoy’s 5A Modified Media (M5AMM) is widely used cor tumor cells. Additionally, modified version of this media contains several folds of amino acids and vitamins compared to the original formulation.
Description
McCoy’s 5A Modified Medium (M5AMM)
Background
McCoy’s 5A Modified Medium (M5AMM) was originally used for Walker Carcinosarcoma 256 Cells. It was subsequently modified to create a new medium known as McCoy’s 5A. Additionally, M5AMM contains several folds of amino acids and vitamins compared to the original formulation. Our McCoy’s 5A covers the main needs for your cell lines, delivers consistency, excellent quality, and the and the full control you need for mammalian cell culture.
MCCOY’S 5A Contains:
- High glucose (3g/L)
- Non-Essential Amino Acids
- Phenol Red
- L-glutamine
Why PurMa Biologics? Why we stand behind our cell culture media:
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.
Application
McCoy’s 5A Medium is widely used for commercial cell line as CHO, HEK 293, HeLa as wellas Jurkat cells. Moreover, PurMaTM McCoy’s Medium contains no proteins, lipids, or growth factors. Therefore, Ham’s F-12 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).
Formulation
complete formulation is available in formulation tab as well as here:McCoy’s 5A Formulation
References
- Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
- Mycoplasmal infection of insect cell cultures. Steiner et al.In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389.
- In vitro culture of postimplantation hamster embryos. Ebron-et al. Reprod Toxicol. 1988;2(1):31-6. doi: 10.1016/s0890-6238(88)80006-6.PMID: 2980399
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | M5AMM- Standard Formulation, M5AMM Without Glutamine, M5AMM -Without Phenol Red, M5AMM -Without Sodium Bicarbonate, M5AMM – Without Glucose, M5AMM – Without Peptone, M5AMM – w/o Glutamine, w/o Phenol Red, M5AMM – Without Phenol Red, Without Sodium Bicarbonate, M5AMM – w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, M5AMM – Low Glucose (1.5 g/L), M5AMM – Low Sodium Bicarbonate (0.35 g/L), M5AMM – Low Sodium Bicarbonate (0.85 g/L), M5AMM -15 mM HEPES (3.6 g/L), M5AMM – 25 mM HEPES (5.9 g/L |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]Formulation
SDS
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