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MEM Alpha With Nucleosides in Earl’s Buffer (MEM-α-N-E)

MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS)

$47.33 – $1077.30

MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside and, furthermore depending on cell types, laboratories use MEM-α-N for growing mammalian cells.

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Description

MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS)

Background

MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside, and the formulation is available below. depending on cell types, laboratories use MEM-α-N for growing mammalian cell for a variety of suspension as well as adherent cells. Eliminating calcium in this media facilitates the growth of cells in suspension cultures.

Ingredients

MEM Alpha Modification with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a modification of MEM.

MEM-α-N-EBSS contains:

  • Non-essential amino acids.
  • Sodium pyruvate.
  • Lipoic acid.
  • Vitamin B12.
  • Biotin and,
  • Ascorbic acid.

PurMa™ MEM-α-N-EBSS includes Earles’ Buffer

As the composition of Earls buffer, it contains:

  1. Calcium chloride
  2. Magnesium sulfate
  3. Potassium chloride
  4. Sodium bicarbonate
  5. Sodium chloride
  6. Sodium Phosphate, monobasic

PurMa™ MEM -α-N-EBSS media also contains:

  • Low level of glucose (1.00 g/ liter)
  • Sodium bicarbonate (2.2 g/L) (if higher than 5% CO2 is used, a higher % of sodium bicarbonate is needed.
  • 2 mM L-glutamine (292 mg/L)’
  • PurMa Biologics manufactures 121 types of MEM -α-N-E

The nucleoside modifies this media  from MEM-α

it contains:

  • Ribonucleosides
  • Deoxyribonucleosides

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.

Application

This media can also be used for keratinocytes, primary rat astrocytes, as well as human melanoma cells. MEM-α-N-EBSS has also been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblast. Additionally, PurMa™ MEM -α-N-EBSS facilitates the growth and maintenance of primary osteoblast cultures. furthermore, this media performs well for isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

Formulation

Click here for complete formulation as well as in ” Formulation Tab”: MEM alpha with Nucleoside Formulation

References

  1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay, L. Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
  2. Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction.  Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)
  3. Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun et al. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

MEM-α-N Standard Formulation, MEM-α-N w/o Glutamine, MEM-α-N w/o Phenol Red, MEM-α-N w/o Sodium Bicarbonate, MEM-α-N w/o Glucose, MEM-α-N w/o Lipoic Acid, MEM-α-N w/o Glutamine, w/o Phenol Red, MEM-α-N w/o Glutamine, w/o Sodium Bicarbonate, MEM-α-N w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α-N w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α-N 15 mM HEPES (3.6 g/L), MEM-α-N 25 mM HEPES (5.9 g/L), MEM-α-N w/o Sodium Pyruvate, MEM-α-N High Glucose (4.5 g/L), MEM-α-N Low Sodium Bicarbonate (0.35 g/L), MEM-α-N Low Sodium Bicarbonate (0.85 g/L), MEM-α-N 15 mM HEPES (3.6 g/L), w/o Glucose, MEM-α-N 25 mM HEPES (5.9 g/L), w/o Glucose, MEM-α-N 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-α-N 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), MEM-α-N 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.85 g/L), MEM-α-N 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.85 g/L), MEM-α-N 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.35 g/L), MEM-α-N 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L), Low Sodium Bicarbonate (0.35 g/L)
Format

Liquid, Powder
Size

1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

MEM-α-N in Earl’s Buffer 

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