MEM α-Modification with Nucleosides MAX (MEM-α-N-MAX) in Hanks’ Buffer

MEM α-Modification with Nucleosides MAX (MEM-α-N-MAX) in Hanks’ Buffer

$55.11$1228.95

MEM α-Modification with Nucleosides MAX in Hanks’ Buffer (MEM-α-N-MAX-HBSS) contains L-Alanyl-L-Glutamine, as well as Nucleosides.   Additionally, MEM-α-N-MAX (HBSS) significantly improves cell viability and growth. We manufacture several types of MEM-α-N-MAX based on your requirements.

Description

MEM α-Modification with Nucleosides MAX in Hanks’ Buffer
(MEM-α-N-MAX-HBSS)

Background

MEM α-Modification with Nucleosides MAX in Hanks’ Buffer (MEM-α-N-MAX-HBSS) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of MEM-α-N-MAX (HBSS) over MEM-α-N (HBSS) are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Consequently, it improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

MEM α-Modification with Nucleosides MAX in Hanks’ Buffer (MEM-α-N-MAX-HBSS) contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of MEM-α-N-MAX (HBSS) based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

PurMa™ MEM-α with nucleosides MAX (MEM-α-N-MAX) is modified from MEM-α-MAX with the following ingredients:

  • Ribonucleosides
  • Deoxyribonucleosides

MEM-α-N-MAX (HBSS) contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

The complete formulation can be found here: MEM alpha-MAX with nucleoside in HBSS Buffer Formulation

References

  1. Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie JA, et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
  2. Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya T, Takahashi K. J Anat. 1987 Jun;152:209-13. PMID: 3654371
  3. Long-term organ culture of hamster cheek pouch mucosa. Mock D, Main JH. J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x. PMID: 820846
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

Formulation

MEM-a-N-MAX with Hank’s Buffer Formulation

Quality Control

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