Advanced Dulbecco′s Modified Eagle′s Medium (A-DMEM)
Advanced Dulbecco′s Modified Eagle′s Medium (A-DMEM) needs 2-5% FBS and provides more consistency.
Description
Advanced Dulbecco′s Modified Eagle′s Medium (A-DMEM)
Background:
PurMaTM Advanced Dulbecco′s Modified Eagle′s Medium (A-DMEM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In Advanced DMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
A-DMEM Contains:
- Advanced Dulbecco′s Modified Eagle′s Medium (A-DMEM) contains all the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- It includes the elements which significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Additionally, A-DMEM is a significant step toward a chemically defined form of this media. Undoubtedly, Chemically Defined DMEM (CD-DMEM) is the outcome of decades of cutting-edge research. CD-DMEM will soon be offered globally to laboratories who are looking for consistency and repeatability among their data.
Formulation: Complete formulation is available here: Advanced DMEM Formulation
References:
- A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. Hu J, Bok D. Mol Vis. 2001 Feb 7;7:14-9. PMID: 11182021
- Protective layer formation on magnesium in cell culture medium. Wagener V, Virtanen S.Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:341-51. doi: 10.1016/j.msec.2016.03.003. Epub 2016 Mar 3.PMID: 27040228
- The viability change of pigskin in vitro. Ge L, Sun L, Chen J, Mao X, Kong Y, Xiong F, Wu J, Wei H. 2010 Jun;36(4):533-8. doi: 10.1016/j.burns.2009.08.001.PMID: 19836142.Additionally
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-DMEM- Standard Formulation, A-DMEM- Without L-Alanyl-L-Glutamine, A-DMEM- Without Phenol Red, A-DMEM- Without Sodium Bicarbonate, A-DMEM- Without Sodium Pyruvate, A-DMEM- Without Glucose, A-DMEM- With Lipoic Acid, A-DMEM- Without L-Alanyl-L-Glutamine, Without Phenol Red, A-DMEM- Without L-Alanyl-L-Glutamine, Without Sodium Bicarbonate, A-DMEM- Without L-Alanyl-L-Glutamine, Without Sodium Pyruvate, A-DMEM- Without L-Alanyl-L-Glutamine, Without Phenol Red, Without Sodium Bicarbonate, A-DMEM- Without L-Alanyl-L-Glutamine, Without Phenol Red, Without Sodium Pyruvate, A-DMEM- Without Sodium Bicarbonate, Without Sodium Pyruvate, A-DMEM- Low Glucose (1.5 g/L), A-DMEM- Low Sodium Bicarbonate (1.5 g/L), A-DMEM- 25 mM HEPES (5.9 g/L), A-DMEM- 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Phenol Red, Without Sodium Pyruvate, A-DMEM- Without Phenol Red, Without Sodium Bicarbonate, A-DMEM- Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), A-DMEM- Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), A-DMEM- Without phenol Red, 15 mM HEPES (3.6 g/L), A-DMEM- Without phenol Red, 25 mM HEPES (5.9 g/L), A-DMEM- Without phenol Red, Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Phenol Red, Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), A-DMEM- Without Phenol Red, Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), A-DMEM- Without Phenol Red, Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Phenol Red, Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Phenol Red, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM- Without Phenol Red, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 1 x 10 L (Powder), 1 X 50 L (Powder), 10 X 1EZ Individually Packed (Powder), 50 X 1L Individually Packed ( Powder), 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml |
Quality Control
[vc-quality-control-tab]SDS
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