Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX)
Dulbecco′s Modified Eagle′s Medium MAX (DMEM-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. DMEM-MAX significantly improves cell viability and growth. We manufacture several types of DMEM-MAX based on your requirements.
Description
Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX)
Background:
Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) is a commonly used media for Mammalian cell culture.
Advantages:
Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) over DMEM:
Higher PH Capacity
Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
Possess longer shelf life.
More importantly, minimizes toxic ammonia build-up.
Significantly improves cell viability and growth.
Remains stable across a wide range of temperatures.
Improves the culture of primary cells.
Mechanism:
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.
In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.
Contents:
Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of DMEM -MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
DMEM-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).
Formulation:
For complete formulation Click here: DMEM MAX Formulation
References:
- A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. Hu J, Bok D. Mol Vis. 2001 Feb 7;7:14-9. PMID: 11182021
- Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19. Ahmado A, et al., Invest Ophthalmol Vis Sci. 2011 Sep 9;52(10):7148-59. doi: 10.1167/iovs.10-6374.PMID: 21743014
- Protective layer formation on magnesium in cell culture medium. Wagener V, et al., Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:341-51. doi: 10.1016/j.msec.2016.03.003. Epub 2016 Mar 3.PMID: 27040228
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | DMEM-MAX Standard Formulation, DMEM-MAX Without Phenol Red, DMEM-MAX Without Sodium Bicarbonate, DMEM-MAX Without Sodium Pyruvate, DMEM-MAX Without Glucose, DMEM-MAX With Lipoic Acid, DMEM-MAX Without Sodium Bicarbonate, Without Sodium Pyruvate, DMEM-MAX Low Glucose (1.5 g/L), DMEM-MAX Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX 25 mM HEPES (5.9 g/L), DMEM-MAX 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX Without Phenol Red, Without Sodium Pyruvate, DMEM-MAX Without Phenol Red, Without Sodium Bicarbonate, DMEM-MAX Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), DMEM-MAX Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), DMEM-MAX Without phenol Red, 15 mM HEPES (3.6 g/L), DMEM-MAX Without phenol Red, 25 mM HEPES (5.9 g/L), DMEM-MAX Without phenol Red, Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX Without Sodium Pyruvate, Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX Without Phenol Red, Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), DMEM-MAX Without Phenol Red, Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), DMEM-MAX Without Phenol Red, Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX Without Phenol Red, Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX Without Sodium Pyruvate, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX Without Sodium Pyruvate, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX Without Phenol Red, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX Without Phenol Red, 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 1 x 10 L (Powder), 1 X 50 L (Powder), 10 X 1EZ Individually Packed (Powder), 50 X 1L EZ individually Packed (Powder), 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml |
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