Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX)

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. DMEM F12-MAX significantly improves cell viability and growth. We manufacture several types of DMEM F12-MAX based on your requirements.

Description

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX)

Background:

Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX) is a commonly used media for Mammalian cell culture.

Advantages:

DMEM F-12 MAX over DMEM:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism:

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents:

  • Dulbecco′s Modified Eagle′s Medium/F-12 MAX (DMEM F12-MAX) contains:
  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of DMEM F12-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

DMEM F12-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation:

Complete formulation is here: DMEM F-12 MAX Formulation

References:

  1.  Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
  2. Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
  3. The nucleic acid of polyoma virus. Smith, J.D., Freeman, G., Vogt, M., et al., (1960) The nucleic acid of polyoma virus. Virology 12:18

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

DMEM F-12 MAX Standard Formulation, DMEM F-12 MAX w/o Phenol Red, DMEM F-12 MAX w/o Sodium Bicarbonate, DMEM F-12 MAX w/o Sodium Pyruvate, DMEM F-12 MAX w/o Glucose, DMEM F-12 MAX w/o Lipoic Acid, DMEM F-12 MAX w/o Phenol Red, w/o Sodium Bicarbonate, DMEM F-12 MAX w/o Sodium Bicarbonate, w/o Sodium Pyruvate, DMEM F-12 MAX w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, DMEM F-12 MAX Low Glucose (1.5 g/L), DMEM F-12 MAX Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 MAX 15 mM HEPES (3.6 g/L), DMEM F-12 MAX 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 MAX w/o Sodium Pyruvate, w/o phenol Red, DMEM F-12 MAX w/o Phenol Red, with 15 mM HEPES (3.6 g/L), DMEM F-12 MAX w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), DMEM F-12 MAX w/o Phenol Red, Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 MAX w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 MAX w/o Sodium Pyruvate, w/o Phenol Red, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 MAX w/o Phenol Red, w/o Sodium Pyruvate, 15 mM HEPES (3.6 g/L), DMEM F-12 MAX w/o Phenol Red, 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 MAX w/o Sodium Pyruvate, with 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 MAX High Glucose (4.5 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L, 10 X 1EZ Individually Packed, 50 X 1EZ Individually Packed

Quality Control

[vc-quality-control-tab]

Formulation

DMEM F-12 MAX Formulation

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