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Earle's Balanced Salt Solution (EBSS)

Earle’s Balanced Salt Solution (EBSS)

(18 customer reviews)

Earle’s Balanced Salt Solution (EBSS) is an isotonic buffer solution. It is however, composed of inorganic salts and carbohydrates as an energy source designed for the short-term maintenance of mammalian cells in a CO2 environment.

SKU: N/A Category: Cell Culture Suited Buffers Tags: Buffers, Calcium and Magnesium Free, Cell Culture, Earle's Buffer, EBSS, Endotoxin Depleted, Phenol Red, Sodium Bicarbonate, Without Phenol Red

Description

Background

Earle’s Balanced Salt Solution (EBSS) is an isotonic buffer solution. It is however, contains inorganic salts and carbohydrates as an energy source designed for the short-term maintenance of mammalian cells in a CO2 environment. The solution additionally provides an ideal environment to preserve the structural and physiological integrity of cells invitro by maintaining pH as well as the balance between intra and extracellular osmotic pressure.

General Features

  • It is cost effective.
  • Cell Culture Suited
  • This buffers maintains the cell integrity of cells or tissues during irrigation, transportation, or dilution.
  • Provides an consistent environment during  the washing and re-suspend cells
  • PurMa Biologics offers EBSS in three pH formats, pH 7.2, 7.4, and pH 7.6.

Specific Features

  • Glucose maintains the intra and extracellular osmotic pressure as well as provides the principal source of energy for cellular metabolism.
  • EBSS Provides water and important inorganic ions necessary for cellular metabolism.
  • This buffer Provides a buffer solution to maintain a medium in physiological pH (7.2-7.6)
  • PurMa Biologics rigorously depletes  Mg2+ and Ca 2+ from the buffers
  • Free of RNAse /DNase
  • Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
  • It is rigorously tested for contamination of endotoxin and mycoplasma.
  • Is manufactured in three pH: 7.2 ± 0.01, pH: 7.4 ± 0.01, and 7.6 ± 0.01 at 25°C.
  • Sterile filtered by 0.22µm membrane (in case of endotoxin-depleted EBSS by 0.1µm)
  • As it is packed under N2, the pH won’t change over time before opening the bottle.

Application

EBSS is especially has an excellent role in protocols requiring the use of consistent conditions such as crosslinking, biotinylation, and fluorescent labeling reactions which require an amine-free buffer.

Formulation

For complete formulation, click here: EBSS Formulation

 

PurMa Biologics Manufactures Several Grades of Buffers

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

We sterilize the buffers by autoclaving the solution. Finally we pack the buffer in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

After adjusting the pH, we finally, sterilize the buffer by  3 times passing through 0.2 µm filters. Additionally,  We pack the buffer under N2 gas  in our Class 100 Aseptic Facility.

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

We sterilize the buffers by autoclaving the solution.  Additionally, we pass the buffer  three times through 0.1 µm filters,  and finally  pack it under N2 gas in our Class 100 Aseptic Facility.

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

After depleting  the endotoxins (see below for more details), we sterilize the buffer by 3 times passing through  0.1 µm filters and pack under N2 gas in our Class 100 Aseptic Facility

The Endotoxin Removing Procedure from Buffer

PurMa Biologics has the most sophisticated endotoxin removal procedure. To remove this pathogen, we pass the buffer  through the PurMa™ Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column,  we covalently conjugate Sepharose 6B  to an endotoxin-specific absorbent. We finally  eliminate the endotoxin residual by passing the unbound fraction three times through 0.1µm filters.

References

  1. Recovery from potentially lethal damage induced by spirohydantoin mustard on 9L cells in vitro. Bertrand M, Deen DF, Hoshino T, Knebel K. Cancer Treat Rep. 1980 Aug-Sep;64(8-9):889-95.
  2. Rotavirus survival in conventionally treated drinking water. Sattar SA, Raphael RA, Springthorpe VS. Can J Microbiol. 1984 May;30(5):653-6. doi: 10.1139/m84-097.
  3. Glucose elevates ornithine decarboxylase expression in Vero cells. Lundgren DW, Prokay SL. J Cell Physiol. 1988 Dec;137(3):469-75. doi: 10.1002/jcp.1041370311.
Parameter Specification
Appearance  Red, clear liquid
pH  7.2 ± 0.1; 7.4 ± 0.1; 7.6 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma  Negative
Suitability  Suitable for mammalian cell culture
Additive  N/A
Indicator  Phenol red
Mycoplasma Detection  Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form  Liquid
Shipping Condition  Room temperature

 

Additional information

Concentration

1X, 10X
Grade

Regular, Cell Culture Suited, Ca and Mg Free, Cell Culture Suited, Ca and Mg Free, Endotoxin Depleted
Phenol Red

With Phenol Red, Without Phenol Red
pH

pH 7.2, pH 7.4, pH 7.6
Size

1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml

18 reviews for Earle’s Balanced Salt Solution (EBSS)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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