Description

Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX)

Background

Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of IMDM-MAX over IMDM are:

• Higher PH Capacity
• Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
• Possess longer shelf life.
• More importantly, minimizes toxic ammonia build-up.
• Significantly improves cell viability and growth.
• Remains stable across a wide range of temperatures.
• Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMa^TM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

Iscove’s Modified Dulbecco’s Medium MAX (IMDM-MAX) contains:

• 5 mM sodium pyruvate
• High glucose (4.5g/L)
• Non-Essential Amino Acids
• 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of IMDM-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
• Phenol Red

IMDM-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

Complete formulation click here: IMDM-MAX Formulation 

References

• Plaque production by the polyoma virus. DULBECCO R, FREEMAN G. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 13669362.
• Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978 Mar 1; 147(3): 923–933. doi: 10.1084/jem.147.3.923 PMCID: PMC2184195
• Development of an infusible-grade solution for non-cryopreserved hematopoietic cell storage. Burger SR, Hubel A, McCullough J. 1999;1(2):123-33. doi:10.1080/0032472031000141250. PMID: 19746589